Filtyp
This thesis, which is based on four original papers, covers different aspects of antibody development. The overall goal of my work has been to obtain an increased understanding of antibody repertoires as they develop in vivo and in vitro. In Paper I-III antibody repertoires was evaluated and used to dissect virus-induced antibody responses and characterize such antibodies. Furthermore, tools for m
A model for foot skin temperature prediction was evaluated on the basis of an experiment on subjects at -10.7 °C (light seated manual work) with winter boots. Insulation of the footwear was measured on a thermal foot model. The predicted temperature stayed higher for the whole exposure period and the difference between the predicted and the measured foot skin temperatures grew proportionally with
The detection of the formyl radical (HCO) is of great interest in the field of combustion research since it can provide information about the local heat release (HR) rate which is a key parameter in the understanding of combustion processes. Unfortunately, due to the low signal level, HCO planar laser-induced fluorescence (PLTF) has so far not been applicable for single-shot imaging, which is nece
People aged 60 or more are the most frequent users of healthcare services. In this age range, however, both frequent and infrequent users can be found. Frequent users have high rates of illnesses. Previous research has found that the frequency may be influenced also by psychological and social factors. The aim of this study was to investigate to what degree such factors add to the explanation of d
Type 1 diabetes mellitus (T1DM) is an autoimmune disorder in which genetics and environmental factors play a role. Among the environmental factors, viruses (especially Coxsackie virus B [CBV]), and among genetic markers, human leukocyte antigen (HLA) DRB1*04-DQA1*0301-DQB1*0302 (DR4-DQ8) and DRB1*03-DQA1*0501-DQB1*0201 (DR3-DQ2), and major histocompatibility complex class I chain-related gene-A (M
Background-Atrial fibrillation (AF) is heterogeneous at the clinical and molecular levels. Association studies have reported that common single-nucleotide polymorphisms in KCNE1 and SCN5A may predispose to AF In this study, we tested the hypothesis that specific AF-associated genotypes confer variation on the appearance of AF assessed by analysis of fibrillatory rate of the atria. Methods and Resu
This paper presents a new fast approach to improving stability in polynomial equation solving. Gröbner basis techniques for equation solving have been applied successfully to several geometric computer vision problems. However, in many cases these methods are plagued by numerical problems. An interesting approach to stabilising the computations is to study basis selection for the quotient space C[
This paper demonstrates the potential of asymmetrical flow field flow fractionation coupled with online multi-angle light scattering, differential refractive index and UV detection for the fractionation and analysis of macromolecules in beer regarding their composition, molar mass (M) and relative concentration. The macromolecules in the liquid and foam of two types of beer, light lager and porter
The Shrinking Generator and the Alternating Step Generator are two of the most well known clock-controlled stream ciphers. We consider correlation attacks on these two generators, based on an identified relation to the decoding problem for the deletion channel and the insertion channel, respectively. Several ways of reducing the decoding complexity are proposed and investigated, resulting in “divi
Radiative lifetime measurements were performed with time-resolved laser-induced fluorescence techniques for 24 levels of Nd II in the energy range 20 500- 32 500 cm(-1). For 17 levels, no previous experimental data exist. These results have allowed the testing of new theoretical calculations with the relativistic Hartree-Fock method taking configuration interactions and core-polarization effects i
Pattern recognition is an integral part of the innate immune system. The human contact system has been shown to interact with the surface of many bacterial and fungal pathogens, and once activated leads to the generation of antimicrobial peptides and the proinflammatory mediator bradykinin. Here we show that apart from these surfaces also neutrophil extracellular traps (NETs) provide a surface tha
In adult mice, the VpreB genes are expressed in bone marrow progenitor (pro-) and precursor (pre-) B cells. As part of the pre-B cell receptor, the proteins are crucial for the proliferation of these cells and consequently normal B lymphocyte development. Using cell lines, we identified a lineage- and developmental-stage-specific VpreB1 enhancer. Here, we analyze its specificity in vivo by generat
HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a molecular complex derived from human milk that kills tumor cells by a process resembling programmed cell death. The complex consists of partially unfolded alpha-lactalbumin and oleic acid, and both the protein and the fatty acid are required for cell death. HAMLET has broad antitumor activity in vitro, and its therapeutic effect has
In this paper we studied carrier drift dynamics in APFO3:PC61BM solar cells of varied stoichiometry (2:1, 1:1, and 1:4 APFO3:PC61BM) over a wide time range, from subpicoseconds to microseconds with a combination of ultrafast optical electric field probing and conventional transient integrated photocurrent techniques. Carrier drift and extraction dynamics are strongly stoichiometry dependent: the s
In 2010, unmet medical care and sexual health counseling needs were assessed among students at a Ugandan University. Unmet medical care need was associated with poor mental health, experience of sexual coercion, and poor self-rated health. Unmet sexual health counseling need was significantly associated with being female, coming from an urban area, low social participation, poor mental health stat
Quantum-dot-sensitized solar cells are emerging as a promising development of dye-sensitized solar cells, where photostable semiconductor quantum dots replace molecular dyes. Upon photoexcitation of a quantum dot, an electron is transferred to a high-band-gap metal oxide. Swift electron transfer is crucial to ensure a high overall efficiency of the solar cell. Using femtosecond time-resolved spect
