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DNA interaction with catanionic vesicles

DNA-cationic liposome complexes as possible vehicles for gene delivery is currently an important issue. In this work, the interaction between DNA and thermodynamically stable, spontaneously formed, catanionic vesicles with a net positive charge is studied. A phase map was drawn for the aqueous system of DNA and positively charged vesicles, composed of CTAB (cetyltrimethylammonium bromide) and SOS

Investigation of the segregative phase separation induced by addition of polystyrene to AOT oil-continuous microemulsions

The effect of different molecular weight polystyrenes (PS) on the phase behavior of sodium 1,4-bis(2-ethylhexyl) sulfosuccinate (AOT)/water/cyclohexane oil-continuous microemulsions was investigated. The surfactant-covered water droplets were treated as a pseudocomponent in the mixture, and ternary polymer/droplet/oil phase diagrams were established as a function of microemulsion droplet radius (2

Complex formation in solutions of oppositely charged polyelectrolytes at different polyion compositions and salt content

The formation of complexes in solutions containing positively charged polyions (polycations) and a variable amount of negatively charged polyions (polyanions) has been investigated by Monte Carlo simulations. The polyions were described as flexible chains of charged hard spheres interacting through a screened Coulomb potential. The systems were analyzed in terms of cluster compositions, structure

Mixing oil and water by a DNA-based surfactant

The self-assembly behavior of a cationic surfactant (dodecyltrimethylammonium) with DNA as counterion in mixtures of water and decanol was investigated. The phase diagram was established and the different regions of the phase diagram characterized with respect to microstructure by H-2 NMR (D2O), small-angle X-ray scattering (SAXS), and other techniques. The DNA-cationic surfactant aggregate is vir

Aspects in Context

The review highlights the new sources for the understanding of the shifting factors that have shaped the establishment and further development of the psychology of religion internationally.

Flight costs and fuel composition of a bird migrating in a wind tunnel

We studied the energy and protein balance of a Thrush Nightingale Luscinia luscinia, a small long-distance migrant, during repeated 12-hr long Eights in a wind tunnel and during subsequent two-day fueling periods. From the energy budgets we estimated the power requirements for migratory flight in this 26 g bird at 1.91 Watts. This is low compared to flight cost estimates in birds of similar mass a

Characterization of the protein-synthesis dependent adaptive acid tolerance response in Lactobacillus acidophilus

Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the

Family structure in the Siberian Jay as revealed by microsatellite analyses

The Siberian Jay (Perisoreus infaustus) lives in resident, territorial family groups outside the breeding season, but does not breed cooperatively. Thereby it offers an opportunity to study the evolution of territorial group living, without confounding effects of reproductive cooperation. During a long-term study in Finland 1974-2000, we observed Siberian Jay group composition in autumn. Using mic

Immune responses to bile-tolerant Helicobacter species in patients with chronic liver diseases, a randomized population group, and healthy blood donors

Bile-tolerant Helicobacter species such as Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of H. pullorum, H. bilis, and H. hepaticus in serum samples from patients with CLD, a randomized population gro

Transepithelial neutrophil migration is CXCR1 dependent in vitro and is defective in IL-8 receptor knockout mice

Neutrophil migration across infected mucosal surfaces is chemokine dependent, but the role of chemokine receptors has not been investigated. In this study, chemokine receptors were shown to be expressed by epithelial cells lining the urinary tract, and to play an essential role for neutrophil migration across the mucosal barrier. Uroepithelial CXCR1 and CXCR2 expression was detected in human urina

Lysozyme-water interactions studied by sorption calorimetry

Hydration of hen egg white lysozyme was studied by using the method of sorption calorimetry at 25, 40, and 50degreesC. Desorption calorimetric measurements were performed at 25 and 40degreesC. The activity of water and partial molar enthalpy of mixing of water were determined as functions of water content. Hydration of lysozyme occurs in four steps: slow penetration of water into the protein-prote

High Myc pathway activity and low stage of neuronal differentiation associate with poor outcome in neuroblastoma.

The childhood cancer neuroblastoma arises in the developing sympathetic nervous system and is a genotypically and phenotypically heterogeneous disease. Prognostic markers of poor survival probability include amplification of the MYCN oncogene and an undifferentiated morphology. Whereas these features discriminate high- from low-risk patients with precision, identification of poor outcome low- and

The amateur scientist's spectrophotometer

This chapter describes the construction and performance of a spectrophotometer that is very easily constructed from a DVD, a digital camera, a lamp and some cardboard, wood, and sheet metal.

Control strategy for the adaptive optics of the Euro50

The scheme presently envisaged for the EURO50 adaptive optics ispresented. The Euro50 adaptive optics will primarily work with laserguide stars (LGSs) and control of either one or two deformable mirrors(SCAO and DCAO respectively), but operation using a natural guide star(NGS) is also foreseen. The point spread function (PSF) for SCAOoperation using a single NGS is evaluated. An algorithm for opti