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A novel high mobility group box 1 neutralizing chimeric antibody attenuates drug-induced liver injury and postinjury inflammation in mice

Acetaminophen (APAP) overdoses are of major clinical concern. Growing evidence underlines a pathogenic contribution of sterile postinjury inflammation in APAP-induced acute liver injury (APAP-ALI) and justifies development of anti-inflammatory therapies with therapeutic efficacy beyond the therapeutic window of the only current treatment option, N-acetylcysteine (NAC). The inflammatory mediator, h

Assessing the viability of probiotic preparations with flow cytometry. A comparison of different methods

Ändrade levnadsvanor i västerländska länder har lett till en ökning av sjukdomar så som IBS, infektioner i mag- och tarmkanal och allergier. Det har dessutom observerats att mikrofloran förändrats och goda bakterier så som Limosilactobacillus reuteri försvunnit. Mag- och tarmkanalen har därmed förlorat sina goda soldater mot patogena fiender med dysfunktionell balans som resultat. Därför är det inThis master thesis work was performed in collaboration with BioGaia AB, a Swedish biotech- company producing probiotic supplements containing the bacteria Limosilactobacillus reuteri. The products provided by BioGaia contain live microorganisms. Therefore, a method suitable for the correct enumeration of living cells is crucial in probiotic samples and today the so-called plate count method is use