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Cyclomaltodextrinases are multidomain and often dimeric proteins from the alpha-amylase family (glycoside hydrolase family 13) which frequently have been very difficult to express in active form in Escherichia coli. To express the soluble form of this type of proteins in larger quantities the expression has to be optimized. We have used and combined two strategies to increase the yield of soluble
Popular Abstract in English N/AAquatic systems worldwide receive large amounts of organic carbon from terrestrial sources. This ‘allochthonous’ organic carbon (AlloOC) affects critical physical and chemical properties of freshwater ecosystems, with consequences for food web structures and exchange of greenhouse gases with the atmosphere. In the boreal region, loadings of AlloOC are particularly high due to leaching from huge or
OBJECTIVES: To determine wild-type minimum inhibitory concentration (MIC) distributions for Mycobacterium tuberculosis, as the background data for defining susceptibility breakpoints are limited. METHODS: We determined wild-type MIC distributions of M. tuberculosis using a 96-stick replicator in Middlebrook 7H10 (7H10) medium for ethionamide (ETH), prothionamide, thiacetazone, cycloserine, rifabut
DIGITAL ANALYSIS OF CARDIAC ACOUSTIC SIGNALS IN CHILDREN Milad El-Segaier, MD Division of Paediatric Cardiology, Department of Paediatrics, Lund University Hospital, Lund, Sweden SUMMARY Despite tremendous development in cardiac imaging, use of the stethoscope and cardiac auscultation remains the primary diagnostic tool in evaluation of cardiac pathology. With the advent of miniaturized and po
In aqueous solutions hydrophobically modified polyethylene glycol (HM-PEG) forms a transient polymer network held together by intermolecular hydrophobic associations. In the present investigation we have used NMR-diffusometry to study how the addition of methylated alpha-cyclodextrin (M-alpha-CD) influences the polymer network. The addit on of M-alpha-CD resulted in an increased mean self-diffusio
The effects of a driver assistance system for keeping safe speed and safe distance (referred to as SASPENCE) on driver behaviour, reactions and acceptance were evaluated in a test carried out in 2006. Twenty test drivers, recruited by ads, drove two times (once with the system off and once with the system on) in real traffic conditions along a 50 km long test route containing urban and rural roads
In this work, the influence of substrate bias voltage on the microhardness, adhesive strength, friction coefficient, and wear rate of AIP Cr2O3 films deposited on AISI 304 stainless steel substrates was investigated systematically. In the meantime, the wear failure mechanism of AIP Cr2O3 films in dry sliding contact was also analyzed and discussed. The results showed that the mechanical properties
Implicit assumptions, approaches, rules and practices in teaching i.e. teaching and learning regimes (TLRs) are produced and reproduced (cf. Trowler & Cooper 2002) in the closest teaching environment, traditionally within a discipline. However, multidisciplinary programs implies that teachers from different disciplines and with different taken for granted assumptions, approaches and rules are Implicit assumptions, approaches, rules and practices in teaching i.e. teaching and learning regimes (TLRs) are produced and reproduced (cf. Trowler & Cooper 2002) in the closest teaching environment, traditionally within a discipline. However, multidisciplinary programs implies that teachers from different disciplines and with different taken for granted assumptions, approaches and rules are
Some 4 billion years ago, nature began the experiment that led to you, that bird on the branch, that branch, and everything else which is alive that you see around you. The generating process of all this splendid life is, of course, evolution. Evolution works not only on the macroscopic scale (think eyes, muscles, wings) but also on the molecular scale (think molecular antennas, nanoscale muscles,The sequence diversity of protein families is a result of the biophysical selection pressures that shaped their evolutionary history. Among the dominant pressures is selection for protein thermostability, which in itself is an attractive target in protein engineering because of its importance for various biopharmaceutical properties, the performance of industrial enzymes, and the ability to design
Research on winter energy management in small vertebrates has focused on the regulation of body mass (BM) within a framework of starvation-predation trade-off. Winter-acclimatized birds exhibit a seasonal increase in both BM and basal metabolic rate (BMR), although the patterns of co-variation between the two traits remain unknown. We studied this co-variation in three different species of wild ti
This paper examines the linkages between cash-crop income and other dimensions of poverty to interrogate assumptions regarding the relationship between agricultural income and poverty alleviation. The analysis treats poverty as a multi-dimensional and socially disaggregated phenomenon. The paper employs a mixed methods approach to case studies of Ghana and Ethiopia to explore two critical issues.
Ultrafiltration can be used to concentrate arabinoxylan isolated from wheat bran. Prefiltration with diatomaceous earth and air sparging during ultrafiltration of the alkaline hemicellulose solution were both found to increase the flux, while nitrogen sparging had no effect. The flux of the untreated solution was 51 L/m2 h, while the flux after prefiltration of the solution with diatomaceous earth
The use of mobility devices, such as walking sticks and rollators, increases during the ageing process. Our aim was to explore how very old single-living Swedish women experience the use of mobility devices over time, in relation to everyday occupation. A multiple case study strategy involving quantitative and qualitative data was used. The findings indicate that the use of mobility devices, rolla
Previous studies have shown that belief in the healthiness of organic food is a strong motive for buying organic. Typically, a positive relation between a nutritionally balanced diet (with respect to fruit, vegetables and meat) and organic consumption is also found. As market shares of organic food are much smaller than those of conventional food, consumers may face a trade-off between buying orga
Birds migrate across the globe on their wings, a behavioural adaptation that allows birds to exploit seasonal environments. Their migrations are ultimately influenced by their ability to fly, a costly but high energy-efficient mode of locomotion that enables birds to travel far distances in a short amount of time. This thesis investigates bird migration from a flight energetic perspective, focusin
Background: Adverse pregnancy outcomes, such as preterm delivery and hypertensive disorders of pregnancy, may be associated with higher future risks of heart failure (HF). However, the comparative effects of different adverse pregnancy outcomes on long-term risk of HF, and their potential causality, are unclear. OBJECTIVES: The authors sought to examine 5 major adverse pregnancy outcomes in relati
At a time of increasing environmental changes and geopolitical tensions, the need for collaboration in the Arctic is greater than ever. Top-down initiatives such as the Arctic Council have contributed to important increased collaboration and science diplomacy. Similarly, bottom-up initiatives have also played a major role in establishing diplomacy among researchers with spin-offs at government lev
This chapter focuses on the experiences of powerlessness among police detectives in a global world. Specifically, it discusses how Danish detectives often feel that certain foreign national criminals get away with their crimes with impunity – here not necessarily meaning that criminals are not caught and sentenced. Rather, what increasingly frustrates Danish detectives are their experiences of how
This thesis has been carried out to investigate a few areas concerning electric and hybrid electric powered land vehicles. The main objective has been to analyze the efficiency of such power trains to compare them with canonical combustion engines, both in a tank-to-wheels basis and a well-to-wheels basis. One of the question formulations is if an electric or plug-in hybrid electric vehicle charge
Increasing global interconnectivity, in terms of our economies, ecological systems and population dynamics, can reduce or amplify the risks posed by climate change. The ongoing Coronavirus 2019 crisis demonstrates how rapidly disasters and risks can cascade and escalate across countries to become a shared problem globally. Climate change impacts are increasingly being recognised not only as a nati
