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Background: Analysis of sialic acid (SA) metabolites in cerebrospinal fluid (CSF) is important for clinical diagnosis. In the present study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) method for free sialic acid (FSA) and total sialic acid (TSA) in human CSF was validated. Methods: The method utilized a simple sample-preparation procedure of protein precipitatio

Large volumes of data from satellite sensors with high time-resolution exist today, e.g. Advanced Very High Resolution Radiometer (AVHRR) and Moderate Resolution Imaging Spectroradiometer (MODIS), calling for efficient data processing methods. TIMESAT is a free software package for processing satellite time-series data in order to investigate problems related to global change and monitoring of veg

In diabetes mellitus (DM), the kidneys are exposed to increased levels of hyperglycemia-induced oxidative stress. Elevated amounts of reactive oxygen species (ROS) are believed to provoke ultrastructural changes in kidney tissue and can eventually result in DM late complications such as diabetic nephropathy. While it is reported that glucagon-like peptide 1 receptors (GLP-1R) are present in the ki

The ST14A cell line was previously derived from embryonic day 14 rat striatal primordia by retroviral transduction of the temperature-sensitive SV40 large T antigen. We showed that cell division and expression of nestin persists at 33°C, the permissive temperature, whereas cell division ceases, nestin expression decreases, and MAP2 expression increases at the nonpermissive temperature of 39°C. In

The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15Δ+P20Δ+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus E

The aim of this study was to determine the influence of insulin-like growth factor binding protein (IGFBP)-1, IGFBP-3, and IGF-I on calcaneal ultrasound parameters in middle-aged and elderly European men. Men aged 40-79 years were recruited from population registers for participation in the European Male Ageing Study (EMAS). Subjects were invited by letter to complete a postal questionnaire and to

Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults and the second most common among children. AML is characterized by aberrant proliferation of myeloid blasts in the bone marrow and impaired normal hematopoiesis. Despite the introduction of new drugs and allogeneic bone marrow transplantation, patients have poor overall survival rate with relapse as the major challeng

Completely homogeneous polyacrylamide-based gels were used for capillary electrochromatography (CEC) of drug enantiomers. Like continuous beds (also called continuous polymer rods, silica rods, monoliths) they do not require frits to support the bed, because it is covalently linked to the capillary wall. Along lifetime is an important feature of the beds. The gel matrices can be prepared in any la

The effects of human recombinant insulin-like growth factor-I (rhIGF-I, 50 ng/ml) on matrix metabolism in the deep flexor tendon from the tendon sheath region of the rabbit were studied in explants cultured for 3 weeks. Tendon segments cultured in medium supplemented with fetal calf serum (FCS) exhibited proliferation of the superficial cell layers. Synthesis of proteoglycan and non-collagen prote

In this study alpha-lactalbumin was converted from the regular, native state to a folding variant with altered biological function. The folding variant was shown to induce apoptosis in tumor cells and immature cells, but healthy cells were resistant to this effect. Conversion to HAMLET (human alpha-lactalbumin made lethal to tumor cells) required partial unfolding of the protein and a specific fat

The international migration of both skilled and unskilled persons from the global South to the North has reached an alarming proportion.This study investigates the underlying factors that informed the decisions of some African students not to return to their countries of origin after graduation from the Swedish Universities. The participants are former and present students of two Swedish Universit

Due to expanding cities and increasing population infrastructure needs to expand in a safe and sustainable way with little effect on the environment and economy. Years of urbanization doesn’t make it easy, no free spaces are available and culture marked buildings are protected. The only alternative left is to construct in layers. Roads and other kinds of infrastructure beneath ground level is ther

Non-exhaust wear emissions from disc brakes affect the air quality in cities throughout the world. These emissions come from the wear of the contact surfaces of both the pads and disc. The tribological and emissions performance of disc brakes strongly depend on the contact surface characteristics of the pads and discs. The surfaces of conventional pads are scorched by heating it to several hundred

Author: Martina Wikås & Anette Johansson Title: Stödcentrum for young crime victims- organizations cooperation for development Supervisor: Carina Tigervall Assessor: Katarina Jacobsson The purpose of this study was to examine how Stödcentrum for young crime victims were perceived by other collaborators that the organization cooperate with and how the collaboration works. The current participa

Neurofilament light chain (NfL) is a promising biomarker of axonal injury across acute and chronic neurodegeneration, which can improve drug discovery and disease monitoring models. Traditional in vivo animal models cannot fully mimic human pathophysiology of neurodegenerative diseases (NDDs), but in vitro models based on human cells solve this problem, reducing the time and cost of drug testing.

Populärvetenskaplig sammanfattning: Sen den industriella revolutionen har koldioxidhalten i atmosfären nästan fördubblats, från 280 ppm till 360 ppm. Detta har skapat stora diskussioner om hur vårt klimat kommer att påverkas. Koldioxid (CO2) är en växthusgas och med detta menas att den absorberar inkommande energi och på så sätt värmer upp vår jord. I denna studie har fokusen lagts på hur vegetatiIncreasing levels of CO2 in the atmosphere may substantially increase net primary productivity (NPP) and thereby increase carbon sequestration by terrestrial ecosystems. Realistic Free Air Carbon Enrichment (FACE) experiments tend to show a NPP enhancement of about 25% if CO2 is elevated by 200 ppm. In this study two generalized vegetation models, the Lund-Postsdam-Jena dynamic generalized vegetat

Detta kandidatarbete undersöker hur utformningen av en produkt kan förenkla vardagshandling och minska beroendet av bil vid inköp av dagligvaror. Arbetet tar sin utgångspunkt i en målgrupp som lever utan bil, ofta i mindre bostäder, och som upplever svårigheter med att integrera spontana eller nödvändiga inköp i en tidspressad vardag mellan jobb, skola och aktiviteter. Syftet har varit att utvecklThis bachelor’s thesis examines how the design of a product can simplify everyday grocery shopping and reduce car dependency. The project focuses on a target group that lives without a car, often in smaller apartments, and faces challenges integrating spontaneous or necessary purchases into a time-pressed daily schedule of work, school, and activities. The aim has been to develop an aid that facil

The supramolecular structures resulting from the complexation between the neutral-cationic block copolymer poly(ethylene oxide)- block-poly(2-(trimethylammonium)ethyl methacrylate iodide) (PEO 114- b-PTMAEMAI 95) and the bile salt sodium deoxycholate (NaDC) were investigated by dynamic light scattering, small-angle X-ray scattering, cryogenic transmission electron microscopy and proton NMR techniq

Experiments using the surface force apparatus have found anomalously long-range interactions between charged surfaces in concentrated salt solutions. Ion clustering has been suggested as a possible origin of this behavior. In this work, we demonstrate that if such stable clusters indeed form, they are able to induce remarkably strong free energy barriers under conditions where a corresponding solu