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Violaxanthin de-epoxidase (VDE) catalyses the conversion of violaxanthin to zeaxanthin at the lumen side of the thylakoids during exposure to intense light. VDE consists of a cysteine-rich N-terminal domain, a lipocalin-like domain and a negatively charged C-terminal domain. That the cysteines are important for the activity of VDE is well known, but in what way is less understood. In this study, w
The effects of human recombinant insulin-like growth factor-I (rhIGF-I, 50 ng/ml) on matrix metabolism in the deep flexor tendon from the tendon sheath region of the rabbit were studied in explants cultured for 3 weeks. Tendon segments cultured in medium supplemented with fetal calf serum (FCS) exhibited proliferation of the superficial cell layers. Synthesis of proteoglycan and non-collagen prote
In this study alpha-lactalbumin was converted from the regular, native state to a folding variant with altered biological function. The folding variant was shown to induce apoptosis in tumor cells and immature cells, but healthy cells were resistant to this effect. Conversion to HAMLET (human alpha-lactalbumin made lethal to tumor cells) required partial unfolding of the protein and a specific fat
The supramolecular structures resulting from the complexation between the neutral-cationic block copolymer poly(ethylene oxide)- block-poly(2-(trimethylammonium)ethyl methacrylate iodide) (PEO 114- b-PTMAEMAI 95) and the bile salt sodium deoxycholate (NaDC) were investigated by dynamic light scattering, small-angle X-ray scattering, cryogenic transmission electron microscopy and proton NMR techniq
Experiments using the surface force apparatus have found anomalously long-range interactions between charged surfaces in concentrated salt solutions. Ion clustering has been suggested as a possible origin of this behavior. In this work, we demonstrate that if such stable clusters indeed form, they are able to induce remarkably strong free energy barriers under conditions where a corresponding solu
Author: Martina Wikås & Anette Johansson Title: Stödcentrum for young crime victims- organizations cooperation for development Supervisor: Carina Tigervall Assessor: Katarina Jacobsson The purpose of this study was to examine how Stödcentrum for young crime victims were perceived by other collaborators that the organization cooperate with and how the collaboration works. The current participa
Title: Climate Change as Experience - A Study on Climate- and Sustainability Communication in Museums Public perception on climate- and sustainability issues is shaped by how they are presented in media and by other communicative actors - like museums. This thesis explores how museum activities can increase public engagement and awareness on climate change and sustainability. The research has bee
The exponential-6 (exp-6) potential is an intermolecular pair potential that is widely used to model fluids at high densities. The path from molecular interaction to equation of state (EOS) for a gas is not straightforward however. Monte Carlo methods and molecular dynamics give accurate results but are too slow for demanding applications like detonation modelling or numerical simulations of react
Growth hormone (GH) has insulin-antagonistic effects, and GH secretion is augmented during fasting and hypoglycemia. In the present study, 10 patients aged 21 to 28 years with childhood-onset GH deficiency (GHD) were studied during a 24-hour fast and a hypoglycemic glucose clamp before and after 9 months of GH replacement. During the 24-hour fast, blood glucose, serum insulin, and serum free fatty
Neurofilament light chain (NfL) is a promising biomarker of axonal injury across acute and chronic neurodegeneration, which can improve drug discovery and disease monitoring models. Traditional in vivo animal models cannot fully mimic human pathophysiology of neurodegenerative diseases (NDDs), but in vitro models based on human cells solve this problem, reducing the time and cost of drug testing.
Populärvetenskaplig sammanfattning: Sen den industriella revolutionen har koldioxidhalten i atmosfären nästan fördubblats, från 280 ppm till 360 ppm. Detta har skapat stora diskussioner om hur vårt klimat kommer att påverkas. Koldioxid (CO2) är en växthusgas och med detta menas att den absorberar inkommande energi och på så sätt värmer upp vår jord. I denna studie har fokusen lagts på hur vegetatiIncreasing levels of CO2 in the atmosphere may substantially increase net primary productivity (NPP) and thereby increase carbon sequestration by terrestrial ecosystems. Realistic Free Air Carbon Enrichment (FACE) experiments tend to show a NPP enhancement of about 25% if CO2 is elevated by 200 ppm. In this study two generalized vegetation models, the Lund-Postsdam-Jena dynamic generalized vegetat
The aim of this study was to determine the influence of insulin-like growth factor binding protein (IGFBP)-1, IGFBP-3, and IGF-I on calcaneal ultrasound parameters in middle-aged and elderly European men. Men aged 40-79 years were recruited from population registers for participation in the European Male Ageing Study (EMAS). Subjects were invited by letter to complete a postal questionnaire and to
The ST14A cell line was previously derived from embryonic day 14 rat striatal primordia by retroviral transduction of the temperature-sensitive SV40 large T antigen. We showed that cell division and expression of nestin persists at 33°C, the permissive temperature, whereas cell division ceases, nestin expression decreases, and MAP2 expression increases at the nonpermissive temperature of 39°C. In
Background: Analysis of sialic acid (SA) metabolites in cerebrospinal fluid (CSF) is important for clinical diagnosis. In the present study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) method for free sialic acid (FSA) and total sialic acid (TSA) in human CSF was validated. Methods: The method utilized a simple sample-preparation procedure of protein precipitatio
Non-exhaust wear emissions from disc brakes affect the air quality in cities throughout the world. These emissions come from the wear of the contact surfaces of both the pads and disc. The tribological and emissions performance of disc brakes strongly depend on the contact surface characteristics of the pads and discs. The surfaces of conventional pads are scorched by heating it to several hundred
Large volumes of data from satellite sensors with high time-resolution exist today, e.g. Advanced Very High Resolution Radiometer (AVHRR) and Moderate Resolution Imaging Spectroradiometer (MODIS), calling for efficient data processing methods. TIMESAT is a free software package for processing satellite time-series data in order to investigate problems related to global change and monitoring of veg
A computational framework for modelling damage-induced softening in fibre-reinforced materials is presented. The main aspect of this framework is the proposed non-local gradient-enhanced continuum damage formulation. At the material level, the elastic constitutive behavior is defined by a hyperelastic functional including a volumetric and an isochoric contribution. The isochoric contribution is su
The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15Δ+P20Δ+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus E
In diabetes mellitus (DM), the kidneys are exposed to increased levels of hyperglycemia-induced oxidative stress. Elevated amounts of reactive oxygen species (ROS) are believed to provoke ultrastructural changes in kidney tissue and can eventually result in DM late complications such as diabetic nephropathy. While it is reported that glucagon-like peptide 1 receptors (GLP-1R) are present in the ki
