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Rock art, trade routes, and languages in prehistoric Amazonia: Exploring correlations through GIS
The aim of this paper is to investigate how Geographical Information System (GIS) mapping of materialized aspects of human culture in pre-conquest Amazonia can increase our understanding of the distribution of indigenous languages and ethno-linguistic entities. The main objective of the project is to build a GIS database for correlating geography, linguistics, material culture (e.g., ceramic style
Bioinformatics and structural characterization of a hypothetical protein from Streptococcus mutans : implication of antibiotic resistance
As an oral bacterial pathogen, Streptococcus mutans has been known as the aetiologic agent of human dental caries. Among a total of 1960 identified proteins within the genome of this organism, there are about 500 without any known functions. One of these proteins, SMU.440, has very few homologs in the current protein databases and it does not fall into any protein functional families. Phylogenetic
DNA binding mechanism revealed by high resolution crystal structure of Arabidopsis thaliana WRKY1 protein
WRKY proteins, defined by the conserved WRKYGQK sequence, are comprised of a large superfamily of transcription factors identified specifically from the plant kingdom. This superfamily plays important roles in plant disease resistance, abiotic stress, senescence as well as in some developmental processes. In this study, the Arabidopsis WRKY1 was shown to be involved in the salicylic acid signaling
Evaluating methods for preventing smoke spread through ventilation systems using fire dynamics simulator
Fires in enclosures equipped with mechanical ventilation remain one of the key issues for fire safety assessment in multifamily homes and industries. Therefore, a wide variation of methods for preventing smoke spread through the ventilation system exist and are applied, in performance-based designs. Through the use of the heating, ventilation and air conditioning (HVAC) model in the fire dynamics
A model inter-comparison study to examine limiting factors in modelling Australian tropical savannas
Financialisation of Built Environments: Urban Governance, Social Geographies, and Sustainability
This paper is about the ways in which financialisation of built environments hasimpacted upon the politics, social constitution and sustainability of cities. We focus primarily on financialisation of housing, and do so from the methodologicalperspective of variegated financialisation: as a generic process that contextually coevolves with related processes such as neoliberalisation and polarisation
Resolving the problem of trapped water in binding cavities : prediction of host–guest binding free energies in the SAMPL5 challenge by funnel metadynamics
The funnel metadynamics method enables rigorous calculation of the potential of mean force along an arbitrary binding path and thereby evaluation of the absolute binding free energy. A problem of such physical paths is that the mechanism characterizing the binding process is not always obvious. In particular, it might involve reorganization of the solvent in the binding site, which is not easily c
A Fast Axonally Transported Protein of the Frog Sciatic Sensory Axons Undergoes Similar Qualitative Changes During Regeneration In Vitro and In Vivo
The adult frog sciatic sensory neurons have been shown to regenerate in vitro. If a crush injury is made at the beginning of culture, regeneration starts after 3.4 days and proceeds at a rate of ∼0.8 mm/day for several days. Two‐dimensional gel electrophoresis was used to study the patterns of radiolabeled, fast axonally transported proteins during the first 7 days of regeneration. Interest was fo
Heterozygous PINK1 p.G411S increases risk of Parkinson's disease via a dominant-negative mechanism
SEE GANDHI AND PLUN-FAVREAU DOI101093/AWW320 FOR A SCIENTIFIC COMMENTARY ON THIS ARTICLE: It has been postulated that heterozygous mutations in recessive Parkinson's genes may increase the risk of developing the disease. In particular, the PTEN-induced putative kinase 1 (PINK1) p.G411S (c.1231G>A, rs45478900) mutation has been reported in families with dominant inheritance patterns of Parkinson's
Detection of a t(1;22)(q23;q 12) translocation leading to an EWSR1-PBX1 fusion gene in a myoepithelioma
Chromosome banding as well as molecular cytogenetic methods are of great help in the diagnosis of mesenchymal tumors. Myoepithelial neoplasms of soft tissue including myoepitheliomas, mixed tumors, and parachordomas are diagnoses that have been increasingly recognized the last few years. It is still debated which neoplasms should be included in these morphologically heterogeneous entities, and the
Necrosis of malignant gliomas after intratumoral injection of 201Tl in vivo in the rat
Fourteen adult Fischer 344 rats were inoculated in vivo unilaterally in the caudate nucleus in the brain with malignant RG 2 glioma cells. By 3 weeks a tumor with a diameter of 3-6 mm normally develops. Ten animals which survived the repeated periods of anesthesia and thallium (Tl) injections (intratumorally three times of 201Tl, 15-23 days after inoculation) showed a prolonged retention of radioa
Dynamics of the caring family
When several individuals simultaneously provide for offspring, as in families, the effort of any one individual will depend on the efforts of the other family members. This conflict of interest among family members is made more complicated by their relatedness because relatives share genetic interest to some degree. The conflict resolution will also be influenced by the differences in reproductive
Down-modulation of the antigen receptor by a superantigen for human B cells
Structural evidence for a ligand coordination switch in liver alcohol dehydrogenase
The use of substrate analogues as inhibitors provides a way to understand and manipulate enzyme function. Here we report two 1 A resolution crystal structures of liver alcohol dehydrogenase in complex with NADH and two inhibitors: dimethyl sulfoxide and isobutyramide. Both structures present a dynamic state of inhibition. In the dimethyl sulfoxide complex structure, the inhibitor is caught in tran
Arthritogenic dsRNA is present in synovial fluid from rheumatoid arthritis patients with an erosive disease course
Viruses may be part of the pathogenesis of rheumatoid arthritis (RA). Double stranded RNA (dsRNA) is a prototypic viral conformation of nucleic acid that is highly arthritogenic in mice. Therefore, we developed an ELISA to detect dsRNA in sera and synovial fluids (SF) in RA patients and in osteoarthritic controls. The developed ELISA recognizes picogram levels of viral or synthetic dsRNA but shows
Development and applications of laser spectroscopic techniques related to combustion diagnostics
Thanks to features as non-intrusiveness combined with high spatial and temporal resolution, various laser diagnostic techniques have during the last decades become of utmost importance for characterization of combustion related phenomena. In the following presentation some further development of the techniques will be highlighted aiming at a) surface temperatures using Thermographic Phosphors, TP
Lactotetraosylceramide, a novel glycosphingolipid receptor for Helicobacter pylori, present in human gastric epithelium
The binding of Helicobacter pylori to glycosphingolipids was examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. In addition to previously reported binding specificities, a selective binding to a non-acid tetraglycosylceramide of human meconium was found. This H. pylori binding glycosphingolipid was isolated and, on the basis of mass spectrometry, proton
Pathogen infection and selection on fertilization success in Cucumis sativus
We investigated whether resistant pollen genotypes can be selected for when the maternal plants are infected and whether infection can result to changes in the pistil nutrient level influencing reproductive outcome. Both resistance and susceptibility come with costs that may affect pre- and post-fertilization reproductive functions. We performed the study by crossing zucchini yellow mosaic virus r
Arterial rupture after microwave-induced hyperthermia and radiotherapy. With reference to two patients treated for recurrence in previously operated and irradiated areas
Two patients who developed frank arterial bleeding after combined microwave-induced hyperthermia and radiotherapy are described. One patient received re-irradiation and hyperthermia for recurrent metastatic neck nodes of a mesopharyngeal carcinoma. Full course radiotherapy had been given 6 years previously and a right-sided radical neck node dissection had been performed 4 months earlier because o
