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Quantitative RT-PCR (QRT-PCR) enables the sensitive and specific detection of mRNA with a small copy number. We used the QRT-PCR method and dual-label analysis of amplification products for the detection of prostate-specific antigen (PSA) mRNA. The QRT-PCR assay employed a PSA-like internal standard (IS) mRNA, which was used to quantify the PSA mRNA copies and to control the variations during the

Context: Offspring of women with gestational diabetes (O-GDM) or type 1 diabetes mellitus (O-T1DM) have been exposed to hyperglycemia in utero and have an increased risk of developing metabolic disease in adulthood. Design: In total, we recruited 206 adult offspring comprising the two fetal hyperglycemic groups, O-GDM and O-T1DM, and, as a control group, offspring from the background population (O

Softwood bark contains a large amounts of extractives—i.e., soluble lipophilic (such as resin acids) and hydrophilic components (phenolic compounds, stilbenes). The effects of the partial removal of water-soluble extractives before acid-catalyzed steam pretreatment on enzymatic digestibility were assessed for two softwood barks—Norway spruce and Scots pine. A simple hot water extraction step remov

Occupancy of cold habitats by evergreen species requires structural modification of photosynthetic organs for stress resistance and longevity. Such modifications have been described at interspecific level, while intraspecific variation has been underexplored. To identify structural and anatomical traits that may be adaptive in cold environments, we studied intraspecific variability of needles of S

In humans, the non-gastric H+/K+ ATPase (ATP1AL1) has previously been shown to be expressed in the epithelia of skin, kidney and colon. In this study we tested the hypothesis that the non-gastric H+/K+ ATPase is localized to the syncytiotrophoblast, the transporting epithelium of the human placenta. Microvillous (MVM) and basal plasma membranes (BM) of the syncytiotrophoblast were isolated from te

A Quality Scorecard (QSC) performance measurement model is proposed to evaluate the quality aspects of an organization in Industry 4.0. To design a QSC for the ‘Fourth Industrial Revolution’, we examined existing measures regarding costs of quality items. The measurement system was refined by simplifying duplicate measures and incorporating quality elements considered in recent international stand

“Alla har en relation till odling och mat, oavsett om man kommer från Somalia, Irak eller Nydala i Malmö.” (Jimenez, 2012). Visst är det så! Att mat är något som vi alla har gemensamt och som förenar människor har varit utgångspunkten för detta arbete. Projektet har resulterat i ett sprudlande matcentrum i Nyhamnen i Malmö. Här kan människor från stadens alla hörn och besökare utifrån samlas krin

The aim of the present study was to take a closer look into the evidence for a theory of prehistoric string manufacture, formulated by the British archaeologist Karen Hardy in the article Prehistoric string theory. How twisted fibers helped to shape the world (Hardy, 2008). A problem with the article, with the relevance and validity of the presented evidence as substance for the theory, was the mi

The smallest of things can make a big impact Nanoparticles (NPs) are very small particles that range from 1-200 nanometers. To put this in perspective, envision a football. A NP is approximately as small compared to a football as the football is to the earth. Considering how small these particles are you can imagine that they have unique abilities to travel throughout living systems. The use of t

The aim of this qualitative study was to investigate and shed light on how care workers perceive their care work and the dilemmas they encounter in the work of adults diagnosed with severe intellectual disabilities. I conducted six semi-structured interviews with care workers working with the study target audience. For my analysis, I have applied the theoretical perspectives ethics of response, pr

Title: Logistic planning on construction sites Author: Anton Grönvall Viktor Baum Supervisor: Radhlinah Aulin, Department of Construction Management, Lund University Faculty of Engineering & Elias Hallberg, Peab bostad AB Examiner:Stefan Olander, Department of Construction Management, Lund University Faculty of Engineering Purpose:The purpose of this rapport is to analyze how different constru

The baobab is among the commonly consumed wild fruits found in the semi-arid regions of sub-Saharan Africa. It has a wide application in food systems and folk medicine and has an advantage of surviving even in harsh environmental conditions. While the fruit pulp is packed with a lot of essential nutrients and has gained much interest in recent past on the international market as a healthy ‘superfo

In order to alleviate the financial burden on the healthcare sector as well as relax its employees’ workload, there is a need to introduce novel tools that automate some of the tasks that today are performed manually. Especially pathology poses a problem with few pathologists, demanding manual labour and unnecessary work on benign tissue. As a response, the DOGS project aims to develop a tool to a