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Pyranose 2-oxidase (P20x) has several proposed biotechnological applications such as a bio-component in biofuel cells or for carbohydrate transformations. To improve some of the catalytic properties of P20x from Trametes multicolor, we selected a semi-rational approach of enzyme engineering, saturation mutagenesis of active-site residues and subsequent screening of mutant libraries for improved ac
The prevalence of allergic disease is continuing to increase worldwide, and it has even become a major concern in public health. Allergic reactions are developed to otherwise harmless substances and may be both life-threatening as well as discomforting for patients in their daily life. Therefore, a more detailed knowledge about how antibodies interact with allergens and effector cells in allergic
Presented here is an engineered protein domain, based on Protein A, that displays a calcium-dependent binding to antibodies. This protein, ZCa, is shown to efficiently function as an affinity ligand for mild purification of antibodies through elution with ethylenediaminetetraacetic acid. Antibodies are commonly used tools in the area of biological sciences and as therapeutics, and the most commonl
The C programming language, while offering high performance and low-level control, is memory-unsafe. This makes it prone to programming errors that can result in serious software vulnerabilities and system instability. In this paper, we investigate the feasibility of transitioning a large code base from C to a memory-safe alternative, specifically Rust, in the context of a large company in telecom
South Korea is attempting to deal with the now-universal issue of disinformation. So why did its draft bill attract such fierce criticism?
I will show that what is respected the most in human reproduction and parenting is not a right to reproduce in the way in which this right is explicitly proposed. The only way in which people can become, and function as, parents without having to submit themselves to anyone else’s judgments and decisions, is by having reproductive sex. Whatever one’s intentions, social status, standard of living,
Spatially and temporally explicit canopy water content (CWC) data are important for monitoring vegetationstatus, and constitute essential information for studying ecosystem-climate interactions. Despite many effortsthere is currently no operational CWC product available to users. In the context of the Satellite ApplicationFacility for Land Surface Analysis (LSA-SAF), we have developed an algorithm
Multiple missiles were fired at Erbil on February 15, killing at least one civil contractor and injuring at least one American national. It is likely that the attack was conducted by an Iranian proxy in order to put pressure on President Biden to lift the sanctions against the country and return to the 2015 nuclear deal.
Information system have become exceedingly important in our daily working lives and by that tightly connected with jobs. Job satisfaction is relatively explored research field which has become very popular in the last decades. But how do information systems affect other aspects of work, and how does our perception of our working life affect our acceptance of information systems is relatively unexp
Every single State has its own rules: legal persons are created based on the domestic laws and States tax them according to their own tax laws. Thus, differences and imperfect legal relationships are inevitable at the international level. The existence of legal entities formed under diverse domestic laws which are not similar to each other leads to disparities and thus, to difficulties to have a c
In this volume of “Lecture Notes in Intelligent Transportation and Infrastructure”, the proceedings of the International Conference “Vision Zero for Sustainable Road Safety in Baltic Sea Region”, which took place in Vilnius, Lithuania, from 5 to 6 December 2018, are presented. The mission of the conference was to promote a more comprehensive approach supporting new ideas, technologies, theories, i
