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This thesis presents four interconnected studies showing methods for characterizing and separating cells based on their acoustic properties, with particular focus on isolating rare cells from blood samples.The first study introduces a technique for single-cell density measurements using microcapillary-adapted density gradient centrifugation. Using fluorescent markers to visualize the density gradi
Background: This study compared levels of physical activity (PA) and sedentary behavior (SB) in adults with and without chronic diseases or multimorbidity, acknowledging sociodemographic factors. Methods: A cross-sectional analysis was conducted with data from 27,890 participants (52% women), aged 50–64, from the Swedish CArdioPulmonary bioImage Study cohort. Over 1500 chronic diseases were includ
Acoustofluidic particle manipulation has attracted considerable interest in biomedical applications due to its non-contact and label-free characteristics. However, existing computational models for predicting particle motion in acoustofluidic systems mainly focus on single-particle behavior, often neglecting particle–particle interactions and the impact of particle distribution on the acoustic and
Stage- and lineage-specific gene expression patterns are controlled by a complex interplay between transcription factors, the epigenetic landscape, and the three-dimensional (3D) structure of the DNA. The 3D structure allows for the formation of DNA loops that juxtaposition distal regulatory elements to the promoters, allowing for tight control of gene expression. Developing a tool to facilitate t
Venetoclax-obinutuzumab (Ven-Obi) is a standard first-line therapy for chronic lymphocytic leukemia (CLL). The impact of age, fitness, and dose reductions remains unclear. We analyzed patients treated with Ven-Obi in the CLL13 and CLL14 trials, excluding patients with TP53 aberrations. Fitness was assessed using the cumulative illness rating scale (CIRS, >6) and creatinine clearance (≤70 mL
Histatins are a family of multifunctional, cationic histidine-rich saliva peptides. The most prominently represented are Histatin 1, Histatin 3, and Histatin 5. Despite considerable similarities in primary structure, the three members are known to display varied antimicrobial properties and healing abilities. This study aims to provide a detailed structural comparison of Histatin 1, Histatin 3, an
In this work, we evaluate the importance of the postmetallization annealing (PMA) temperature on the performance of HfxZr1−xO2-based ferroelectric tunnel junctions (FTJs). Our results indicate a significant difference in tunneling electroresistance (TER) ratio and endurance, depending on the PMA temperature despite negligible variations in remanent polarization. We conclude that the minimization o
Laser-induced Breakdown Spectroscopy (LIBS) is widely applied to the quantitative measurement of gas composition concentrations. However, studies on the effect of gas temperature on LIBS are still lacking. Here, we investigated the effect of gas temperature on LIBS in terms of composition measurements in an air flow field. The air flow field was heated using a resistance wire to different gas temp
Si1−xGex nanowires (NWs) were prepared by gold-supported chemical vapor deposition (CVD) using a single-source precursor with preformed Si–Ge bonds. Besides the tamed reactivity of the precursor, the approach reduces the process parameters associated with the control of decomposition characteristics and the dosing of individual precursors. The group IV alloy NWs are single crystalline with a const
Heterotopic mineralization entails pathological mineral formation inside soft tissues. In human tendons mineralization is often associated with tendinopathies, tendon weakness and pain. In Achilles tendons, mineralization is considered to occur through heterotopic ossification (HO) primarily in response to tendon pathologies. However, refined details regarding HO deposition and microstructure are
SF3B1 is the most mutated splicing factor (SF) in myelodysplastic syndromes (MDSs), which are clonal hematopoietic disorders with variable risk of leukemic transformation. Although tumorigenic SF3B1 mutations have been extensively characterized, the role of “non-mutated” wild-type SF3B1 in cancer remains largely unresolved. Here, we identify a conserved epitranscriptomic program that steers SF3B1
Purpose: To demonstrate the feasibility and accuracy of chemical shift–encoded imaging of the fatty acid composition (FAC) of human bone marrow adipose tissue at 7 T, and to determine suitable image-acquisition parameters using simulations. Methods: The noise performance of FAC estimation was investigated using simulations with a range of inter-echo time, and accuracy was assessed using a phantom
The paper presents novel studies on the impact of different ignition control parameters on combustion stability and spark plug wear. First, experimental results from a 32.4-liter biogas fueled large bore single cylinder spark ignition engine are discussed. Two different ignition systems were considered in the experiment: a DC inductive and an AC capacitive. The spark plugs used in the experiment w
Background Abdominal aortic aneurysm (AAA) is a vascular disease with a mortality rate of >80% if ruptured. Mitochondrial dysfunction has been previously implicated in AAA pathogenesis. In this study, we aimed to characterize the mitochondrial genetic landscape in AAA. Methods and Results Whole mitochondrial genome sequencing and bioinformatics analysis were performed in comorbidity matched 48 cas
Hematopoietic stem cells (HSCs) are rare cells on top of the differentiation hierarchy of hematopoiesis. HSCs are unique in their combined capacity to differentiate into all mature blood lineages and self-renew to maintain the HSC pool. Based on classical models of hematopoiesis in mouse, the self-renewal potential of HSCs is gradually and step-wise lost during the transition from long term (LT)-H
OBJECTIVE: To determine biological variation of the aggrecanase-generated aggrecan ARGS neoepitope in serum (sARGS) and synovial fluid (sfARGS) within and between patients with osteoarthritis (OA) or anterior cruciate ligament (ACL) injury.DESIGN: Matched samples of serum and synovial fluid were available, as parts of clinical trials, from i) 16 subjects with early-stage OA on 8 occasions over 1 y
The plasma proteome can provide a deeper understanding of the pathophysiology of different diseases and is a key source for detection of new biomarkers. Proximity extension assay (PEA), developed by Olink Proteomics (Uppsala, Sweden), detects proteins by using pairs of antibodies that are linked to oligonucleotides; upon target binding, the probes anneal when in proximity and the oligonucleotides
BACKGROUND & AIMS: Gallstones (cholelithiasis) constitute a major health burden with high costs related to surgical removal of the gallbladder (cholecystectomy), generally indicated for symptomatic gallstones. The association between gallstones and cholecystectomy and kidney cancer is controversial. We comprehensively investigated this association, considering age at cholecystectomy and time f
PURPOSE: The Swedish Tattoo and Body Modifications Cohort (TABOO) cohort was established to provide an infrastructure for epidemiological studies researching the role of tattoos and other body modifications as risk factors for adverse health outcomes. It is the first population-based cohort with detailed exposure assessment of decorative, cosmetic, and medical tattoos, piercing, scarification, hen
