Filtyp
Following the theme of this monograph, this paper discusses a dialectic we perceive to subsist between meaningful use and reflection upon use. This dialectic between experiencing use and reflecting upon experiencing use (or thinking, and thinking about thinking) may be considered in the following way. Each of these elements is subject to change. As reflection triggers change in use, and such chang
We develop a density functional theory for infinitely long flexible and semiflexible polymers, as an appropriate simplification of the corresponding treatment of finite chains. We demonstrate that very long chains sometimes are required to reproduce even qualitatively correct limiting behaviors.
A set of new algorithms and software tools for automatic protein identification using peptide mass fingerprinting is presented. The software is automatic, fast and modular to suit different laboratory needs, and it can be operated either via a Java user interface or called from within scripts. The software modules do peak extraction, peak filtering and protein database matching, and communicate vi
Antibody-based microarrays is a rapidly evolving technology that has gone from the first proof-of-concept studies to more demanding proteome profiling applications, during the last years. Miniaturized microarrays can be printed with large number of antibodies harbouring predetermined specificities, capable of targeting high- as well as low-abundant analytes in complex, nonfractionated proteomes. C
The mechanisms by which gestational infections, blood incompatibility, birth weight, mother's age and other prenatal or neonatal events increase the risk for type 1 diabetes are not understood. Studies so far have been retrospective, and there is a lack of population-based prospective studies. The possibility identifying children at type 1 diabetes risk among first-degree relatives has resulted in
We present a software solution that enables faster and more accurate data analysis of 2DE/MALDI TOF MS data. The software supports data analysis through a number of automated data selection functions and advanced graphical tools. Once protein identities are determined using MALDI TOF MS, automated data retrieval from online databases provides biological information. The software, called 2DDB, redu
A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experime
This study investigates the size and power properties of three multivariate tests for autocorrelation, namely portmanteau test, Lagrange multiplier (LM) test and Rao F-test, in the stable and unstable vector autoregressive (VAR) models, with and without autoregressive conditional heteroscedasticity (ARCH) using Monte Carlo experiments. Many combinations of parameters are used in the simulations to
The time-dependent traveling salesman problem (TDTSP) is a variant of TSP with time-dependent edge costs. We study some restrictions of TDTSP where the number of edge cost changes are limited. We find competitive ratios for online versions of TDTSP. From these we derive polynomial time approximation algorithms for graphs with edge costs one and two. In addition, we present an approximation algorit
We investigate the light emission from dipolar emitters located within nanoparticle antennas. It is found that the enormous emission enhancement can reach nearly a million fold. For multinanoparticle antennas, the polarization of the emissions strongly depends on the geometry of the antennas, the emitted wavelengths, and the dielectric functions of surrounding media. It is shown that a polarizatio
Molecular imprinting is a versatile technique providing functional materials able to recognize and in some cases respond to biological and chemical agents of interest. In contrast to biological antibodies, the best known receptors derived from biological combinatorial processes, molecularly imprinted polymers (MIPs) are obtained by template-directed synthesis. Thus, molecular imprinting can more p
Prevention of incorporation of dUTP into DNA is essential for maintenance of the genetic information. Prompt and specific removal of dUTP from the nucleotide pool, as expedited by the ubiquitous enzyme dUTPase, is therefore required for full viability in most biological systems. Conserved structural features perpetuate specificity in choice of substrate, which is crucial as hydrolysis of the struc
Background: Electrocardiogram-derived grades of ischemia at the time of patient presentation with acute myocardial infarction have proved useful in predicting the salvageability by reperfusion therapy, final infarct size, severity of left ventricular dysfunction, and short- and long-term prognosis. Subjects and Methods: The Sclarovsky-Birnbaum Ischemia Grading System based on the relation between
