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Assessing the viability of probiotic preparations with flow cytometry. A comparison of different methods

Ändrade levnadsvanor i västerländska länder har lett till en ökning av sjukdomar så som IBS, infektioner i mag- och tarmkanal och allergier. Det har dessutom observerats att mikrofloran förändrats och goda bakterier så som Limosilactobacillus reuteri försvunnit. Mag- och tarmkanalen har därmed förlorat sina goda soldater mot patogena fiender med dysfunktionell balans som resultat. Därför är det inThis master thesis work was performed in collaboration with BioGaia AB, a Swedish biotech- company producing probiotic supplements containing the bacteria Limosilactobacillus reuteri. The products provided by BioGaia contain live microorganisms. Therefore, a method suitable for the correct enumeration of living cells is crucial in probiotic samples and today the so-called plate count method is use

A novel high mobility group box 1 neutralizing chimeric antibody attenuates drug-induced liver injury and postinjury inflammation in mice

Acetaminophen (APAP) overdoses are of major clinical concern. Growing evidence underlines a pathogenic contribution of sterile postinjury inflammation in APAP-induced acute liver injury (APAP-ALI) and justifies development of anti-inflammatory therapies with therapeutic efficacy beyond the therapeutic window of the only current treatment option, N-acetylcysteine (NAC). The inflammatory mediator, h

From Genotypes of Immunoglobulin Constant Heavy G Chains (Fcγ) (GM) Genes (IGHG) to Phenotypes in Childhood Asthma.

Background: IgE-mediated allergy is associated with immunoglobulin heavy constant G chain (Fcγ) (GM) genes (IGHG) on chromosome 14q32.3. Investigation of the alternative GM allotypes of γ3, γ1 and γ2 chains has disclosed new structural and functional IgG subclasses and B-cell variants, with possible effects on childhood asthma. Objective: To investigate different IGHG (GM) gene complexes in a

Binding of monoclonal IgM rheumatoid factor to streptococci via the antibody combining site

Radiolabelled monoclonal IgM rheumatoid factors, four from patients with type II essential cryoglobulinaemia and one originating from a patient with rheumatoid arthritis, were tested for binding to group A, B, C and G streptococci and Escherichia coli. Two of the preparations exhibited different binding patterns for the streptococci, whereas the remaining three were not reactive. The binding of on

Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). In this thesis, we present different metal binding tags, and their af

Utvc21 ht 2017 schedule 2017-09-29

BIDS UTVC21 Lectures and seminars 2017 (Updated 2017-09-29) Date Time Room Theme Activity Group Lecturer 30/8 10-12 Världen Meet Your Programme Coordinator Lec 1/1 AF 30/8 13-15 Världen Course Introduction Lec 1/1 TAX 1/9 10-12 Världen Introduction: questioning development Potter Ch. 1; Allen et al Ch. 1-2 Lec 1/1 YM 1/9 13-15 Världen Introduction: history and development Allen Ch. 1; Allen et al

https://www.keg.lu.se/sites/keg.lu.se/files/utvc21_ht_2017_schedule_2017-09-29.pdf - 2026-04-25

Early risk prediction in idiopathic versus connective tissue disease-associated pulmonary arterial hypertension : call for a refined assessment

Despite systematic screening and improved treatment strategies, the prognosis remains worse in patients with connective tissue disease-associated pulmonary arterial hypertension (CTD-PAH) compared to patients with idiopathic/hereditary pulmonary arterial hypertension (IPAH). We aimed to investigate differences in clinical characteristics, outcome and performance of the European Society of Cardiolo

Geop04 schema 2016-03-29

Schema för GEOP04 Miljögeologisk markundersökning, 15 hp VT2 2016 Kursansvariga: Håkan Rosqvist hakan.rosqvist@tyrens.se, Per Sandgren per.sandgren@geol.lu.se Lärare från geologiska institutionen: PS = Per Sandgren, professor BS = Britta Smångs, bibliotekarie, geobiblioteket CS = Charlotte Sparrenbom, universitetslektor DH = Dan Hammarlund, professor Externa lärare: SA = Stefan Andersson, miljöför

https://www.geologi.lu.se/sites/geologi.lu.se/files/geop04_schema_2016-03-29.pdf - 2026-04-25

Prekonceptionell halsa 20181218

Prekonceptionell hälsa och rådgivning Kvinnor och mäns levnadsvanor inför graviditet påverkar fostret och det blivande barnets hälsa. Under våren 2019 ges en utbildning i Prekonceptionell hälsa och rådgivning vid Lunds universitet. Utbildningen är den första kursen i Sverige inom prekonceptionell hälsa, vilket gör den unik i sitt slag. Den riktar sig till barnmorskor generellt, och i synnerhet til

https://www.medicin.lu.se/sites/medicin.lu.se/files/prekonceptionell_halsa_20181218.pdf - 2026-04-25

Vehicle driving cycle simulation of a pneumatic hybrid bus based on experimental engine measurements

In the study presented in this paper, a vehicle driving cycle simulation of the pneumatic hybrid has been conducted. The pneumatic hybrid powertrain has been modeled in GT-Power and validated against experimental data. The GT-Power engine model has been linked with a MATLAB/simulink vehicle model. The engine in question is a single-cylinder Scania D12 diesel engine, which has been converted to wor

Proton reduction by phosphinidene-capped triiron clusters

Bis(phosphinidene)-capped triiron carbonyl clusters, including electron rich derivatives formed by substitution with chelating diphosphines, have been prepared and examined as proton reduction catalysts. Treatment of the known cluster [Fe3(CO)9(µ3-PPh)2] (1) with various diphosphines in refluxing THF (for 5, refluxing toluene) afforded the new clusters [Fe3(CO)7(µ3-PPh)2(κ2-dppb)] (2), [Fe3(CO)7(µ

A novel multiplex fluorescent-labeling method for the visualization of mixed-species biofilms in vitro

In nature, bacteria usually exist as mixed-species biofilms, where they engage in a range of synergistic and antagonistic interactions that increase their resistance to environmental challenges. Biofilms are a major cause of persistent infections, and dispersal from initial foci can cause new infections at distal sites thus warranting further investigation. Studies of development and spatial inter