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The scFv antibody towards the Burkholderia pseudomallei exotoxin was previously constructed by phage display and exhibited good specificity towards the exotoxin. We report here the optimization of the scFv expression in an E. coli expression system. Four different E. coli strains (ER2537, TG1, HB2151, and XL1-Blue) were examined for optimal expression of the scFv protein. Two types of carbon sourc

Lipase-catalyzed transesterification of high-oleic-acid rapeseed oil with stearic acid or methyl stearate was investigated. High yields of 1,3-distearoyl-2-monooleyl glycerol (36%) and 1(3)-2-dioleyl-1(3)-monostearoyl glycerol (27%) with small incorporation of stearic acid in the 2-position were obtained by using lipase from Rhizopus arrhizus immobilized on polypropylene powder and ethyl stearate

#039. A Holy Person Falls into the Nile as a Pelican, for clarinet and horn (1997). Premiere: Michelle Doyle-Ronkin, clarinet; Jeremy Ronkin, horn in Green Lake, Wisconsin; 1998_02_13

In this work, an automated downstream process for the purification and formulation of a recombinant protein was integrated at lab scale in a single chromatography unit. The purification chain consists of three bind-and-elute chromatography columns, a flow-through membrane chromatography step, and a final ultrafiltration-diafiltration (UFDF) step to concentrate and formulate the sample. An integrat

The first synchrotron radiation flow linear dichroism spectra are reported. High-quality spectral data can be collected from 450 nm down to 180 nm in contrast to the practical cutoff of approximately 200 nm on benchtop instruments. State-of-the-art microvolume capillary Couette flow linear dichroism was successfully ported to a synchrotron radiation source. The sample volume required is < 50 micro

The aim of this report is to establish recommendations for reviewing, reporting, and conducting research during the post-resuscitation period in hospital. It defines data that are needed for research and more specialised registries and therefore supplements the recently updated Utstein template for resuscitation registries. The updated Utstein template and the out-of-hospital "Chain of Survival" d

This paper analyses the underlying reasons why innovators do not apply for trademarks for all of their valuable inventions. Using a unique database of UK innovations linked to innovative firms, the empirical analysis highlights the many ways that firms can alleviate information asymmetries and the constraints imposed by collaborative innovation without taking recourse to trademarks. When informati

In response to growing social injustices in cities, bottom-up initiatives are increasingly recognised as sites of resistance and experimentation. I examine how the PARKS project in Hamburg, a temporary, collectively governed green space, challenges neoliberal urban development and envisions socially just green spaces. Adopting a case study approach, I draw on semi-structured interviews, participan

The increase in contact resistance within battery systems remains poorly understood, despite being one of the primary contributors to battery degradation. This project aims to investigate this phenomenon by modeling the electrical interface as a contact between a pin and a busbar. The main goal is to build a DOE and machine learning framework, using Bayesian optimization to efficiently explore the

This thesis investigates how startups in Tier 2 European cities achieve sustainable growth by engaging with their local entrepreneurial ecosystems. While prior research has largely focused on major capital cities, this study aims to understand how emerging urban canters with less mature infrastructures can still support high-growth entrepreneurial activity. A qualitative research design was employ

This study investigates the corrosion behavior of a magnesium-zinc (Mg-Zn) alloy in a simulated body-like environment, with the aim of optimizing its degradation rate for biodegradable medical implant applications. These implants are designed to support bone healing and then gradually degrade once no longer needed, thereby eliminating the need for additional surgery. Magnesium alloys are promising

In the evolving landscape of digital entrepreneurship, social media influencers (SMIs) have emerged as strategic actors navigating complex stakeholder environments to sustain long-term business viability. While existing literature extensively addresses branding, authenticity, and audience engagement, it often overlooks the strategic coordination required to manage stakeholder relationships across

Electricity grids are essential to industrialized societies, yet they are increasingly unable to meet the dual demands of increased electrification and electricity system decarbonization. Smart grids offer a solution to these challenges by integrating information and communication technologies into the physical grid. However, despite their potential, smart grid implementations often fail to delive

This thesis examines how Sweden’s new development policy, Development for a New Era, reconfigures the agency of its civil society organizations (CSOs). The policy marks a major shiftin Sweden’s foreign aid landscape, moving from a human rights– and needs-based approach to one driven by strategic, trade, and migration interests. Drawing on a qualitative analysis of interviews with CSO staff and med

After the Higgs boson’s discovery precision searches for Beyond the Standard Model physics has become a new frontier in fundamental physics research. In extensions to the Standard Model, large combinatorics arising due to the many possibly involved particles in Feynman diagrams stands in the way of performing precision calculations of what such extensions predict. This work investigates the feasib