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Long DNA isolation and imaging using lateral displacement arrays integrated with DNA combing

We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dy

Selective (E)valuations : The Production of Opacity and Transparency of Values in the Swedish Meat Supply Chain

(E)valuative technologies such as standards and conventionalized (e)valuative practices, some including the use of technological devices, govern the production of food in the global north. Food supply chains consist of multiple interrelated markets, where goods are (e)valuated to distinguish their qualities and prices. Qualities and prices that are transformed as the raw materials move through the

Markov modeling of peptide folding in the presence of protein crowders

We use Markov state models (MSMs) to analyze the dynamics of a β-hairpin-forming peptide in Monte Carlo (MC) simulations with interacting protein crowders, for two different types of crowder proteins [bovine pancreatic trypsin inhibitor (BPTI) and GB1]. In these systems, at the temperature used, the peptide can be folded or unfolded and bound or unbound to crowder molecules. Four or five major fre

Tunable separation and DNA manipulation in metal coated pillar arrays

Implementing electrically connected metal-coated posts in a Deterministic Lateral Displacement (DLD) device and applying electric fields, we use electrokinetics to achieve tunable particle separations and to trap and manipulate DNA. The strength of DLD stems from its typically binary behavior. Particles move in one out of two trajectories based on their effective size. For particles that are close

ACouWash : A standalone instrument for the washing, separation and enrichment of cells.

We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing,

Heart University : a new online educational forum in paediatric and adult congenital cardiac care. The future of virtual learning in a post-pandemic world?

Online learning has become an increasingly expected and popular component for education of the modern-day adult learner, including the medical provider. In light of the recent coronavirus pandemic, there has never been more urgency to establish opportunities for supplemental online learning. Heart University aims to be "the go-to online resource" for e-learning in CHD and paediatric-acquired heart

Återanpassning efter coronakrisen: Nytt liv för stadskärnan

Coronapandemin har inneburit ett hårt slag för café- och restaurangbranschen då kunderna i stor utsträckning uteblir, dels på grund av rädsla för smitta, dels på grund av uppmaningar från myndigheterna om social distansering. I branschen ärmånga småföretagare utan större ekonomisk buffert. Innan krisen är över kommer sannolikt många företag att ha tvingats lägga ned verksamheter (DN, 2020). Vi för

Grannen vet bäst - Faktorer som påverkar hushållens val av energiformer

In today’s society people are overwhelmed with information about different kinds of heating systems. The purpose of this study is to examine the factors that impact households when they make decisions on heating systems, and also how households value different types of information on the subject. Four Swedish municipalities were chosen for closer examination. Municipal energy advisors and represe

Renewable energy, energy security and resilient societies

Abstract in UndeterminedSometimes energy security is used as an advocacy for renewable energy systems. Although renewable energy systems can surely have such characteristics they will not per se solve all types of security problems, and new problems will most certainly arise. In this paper, energy security aspects of renewable energy systems are analysed taking the starting point in a broad typolo