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Standard ZYMO DNA/RNA miniprep extraction protocol written in a user friendly way to reduce in-process-decisions and process time

Conservation grazing is widely used to manage open ecosystems, but inconsistent definitions and inadequate reporting of grazing patterns hinder effective communication among researchers, practitioners and policymakers, limiting the understanding and success of conservation grazing regimes. We demonstrate detailed measurement of the grazing pattern (comprising timing, intensity, duration and freque

To ensure that cities and urban ecosystems support human wellbeing and overall quality of life we need conceptual frameworks that can connect different scientific disciplines as well as research and practice. In this perspective, we explore the potential of a traits framework for understanding social-ecological patterns, dynamics, interactions, and tipping points in complex urban systems. To do so

Interactions between microbiomes and metabolites play crucial roles in the environment, yet how these interactions drive greenhouse gas emissions during ecosystem changes remains unclear. Here we analysed microbial and metabolite composition across a permafrost thaw gradient in Stordalen Mire, Sweden, using paired genome-resolved metagenomics and high-resolution Fourier transform ion cyclotron res

Slight modifications to the manufacturer’s protocol (Qiagen #12966-10, now discontinued and replaced with #12988-10) enabled the successful extraction of high-quality DNA and RNA from permafrost peat samples. Integrating the DNA and RNA purification steps immediately after the extraction process helped streamline the workflow, resulting in consistent quality nucleic acids suitable for downstream s

Soil microorganisms are pivotal in the global carbon cycle, but the viruses that affect them and their impact on ecosystems are less understood. In this study, we explored the diversity, dynamics, and ecology of soil viruses through 379 metagenomes collected annually from 2010 to 2017. These samples spanned the seasonally thawed active layer of a permafrost thaw gradient, which included palsa, bog

Permanent ponds represent key landscape components that support biodiversity and supply various ecosystem services. Notably, the export of aquatic subsidies to land via emerging insects, may significantly influence terrestrial food webs. Various factors, including eutrophication, can influence these exports. Polyunsaturated fatty acids (PUFA), which greatly enhance consumer fitness, are among the

The European Green Deal recognises the importance of addressing the environmental challenges posed by agriculture to meet the global biodiversity and climate agendas. Environmental policymaking is needed to drive and speed up sustainability transitions in agriculture because the environmental consequences of agricultural activities are not generally reflected in production costs and lead to market

Arctic tundra ecosystems store a significant proportion of the global soil organic carbon (C). However, warming-induced shrub encroachment and reindeer (Rangifer tarandus L.) grazing regimes promoting graminoid vegetation may strongly influence tundra soil C stability. Here, we studied how reindeer grazing intensity and experimental warming affect soil C stabilization in a tundra ecosystem. We hyp

Understanding and addressing the complex societal sustainability challenges requires applying novel approaches, devising innovative assessment frameworks, and adopting methods and tools from other research fields. Dr Jagdeep Singh has been conducting inter- and trans-disciplinary research on these challenges impacting academia and society. He has pursued university degrees in Electrical Engineerin

The phyllosphere, particularly the leaf surface of plants, harbors a diverse range of microbiomes that play a vital role in the functioning of terrestrial ecosystems. However, our understanding of microbial successions and their impact on functional genes during plant community development is limited. In this study, considering core and satellite microbial taxa, we characterized the phyllosphere m

Social behavior is observed in a wide range of species, and its potential benefits include protection from predators, enhanced feeding, and reproductive opportunities. Experimental approaches using artificial selection on sociability can answer fundamental questions about the evolution of social behavior, such as: Can sociability evolve by artificial selection? Is aggressiveness associated with so

We apply causal loop diagrams (CLD) to picture how complex societal scenarios can be understood in terms of interdependent drivers and mechanisms between actors from the public and private sectors respectively. And we show how un-sustainable scenarios can be understood in terms of insufficient balancing feed-back in the system. We apply the methodology to picture such imbalances as funda- mental d

Political institutions are keen to use the best available scientific knowledge in decision-making. For environmental policy, relevant scientific evidence can be complex and extensive, so expert judgment is frequently relied upon, without clear links to the evidence itself. We propose a new transparent process for incorporating research evidence into policy decisions, involving independent synopsis

When designing compensation payments for biodiversity conservation measures setting appropriate incentives so that landowners select spatially connected land parcels for conservation is of key importance for many species. The proposition of an agglomeration bonus, where landowners receive a homogeneous payment and a bonus on top if the spatial allocation of conserved land benefits biodiversity, ha

Croplands are vital ecosystems for human well-being and provide important ecosystem services such as crop yields, retention of nitrogen and carbon storage. On large (regional to global)-scale levels, assessment of how these different services will vary in space and time, especially in response to cropland management, are scarce. We explore cropland management alternatives and the effect these can

Context Agricultural transformation and increased land use intensity often lead to simplified landscapes and biodiversity loss. For animals, one possible mechanism underpinning biodiversity loss in agricultural landscapes is the disruption of movements. The disruption of movements may explain, for example, why butterfly communities in agricultural landscapes are often dominated by generalist speci

Information on the ecological traits of species might improve predictions of climate-driven range shifts. However, the usefulness of traits is usually assumed rather than quantified. Here, we present a framework to identify the most informative traits, based on four key range-shift processes: emigration of individuals or propagules away from the natal location; the distance a species can move; est

Global land-use change has drastic consequences for biodiversity leading to losses of ecological functioning, ecosystem services and human well-being. While species dependent on undisturbed natural habitat are most affected by conversion to agriculture, even populations of disturbance-tolerant species can be endangered in landscapes dominated by high-input mono-cultural cropping systems. This has