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A method for detection and genotyping of genital Chlamydia trachomatis infections based on omp1 gene amplification and sequencing was developed. DNA was extracted from urogenital or urine samples using a Chelex-based method, and an approximately 1,100-bp-long fragment from the omp1 gene was directly amplified and sequenced. Genotyping was performed by BLAST similarity search, and phylogenetic tree

“The eyes lead the body.” A good visual environment is important for performance and wellbeing. Unsatisfactory visual conditions often induce a strained posture, more or less unconsciously, in an effort to improve vision. The requirements for the visual environment in an operating theatre are high. Surgeons or assistants who are having visual problems can induce treatment hazards. The purpose of t

In this paper we develop an Enterprise Systems Implementation Methodology in Action framework. The framework is grounded in contemporary views on methodology in action (theoretical grounding) and enhanced by an empirical study (empirical grounding). The notion of Implementation Methodology in Action, as articulated in this paper, integrates two complementary views that conceptualise a methodology

Capacity development for disaster risk reduction is an important process to substantially reduce disaster losses, which threaten sustainable development and the achievement of the Millennium Development Goals. This paper presents a theoretical framework with seven elements for capacity development for disaster risk reduction that has been tested in practise with noteworthy results. The seven eleme

Popular Abstract in Swedish Intensifieringen av jordbruket under de senaste sextio åren har resulterat i en dramatisk produktionsökning av framförallt spannmål. Denna typ av produktion, speciellt det mest intensiva s.k. industrijordbruket, har samtidigt också resulterat i en ohållbar degradering av ekosystemen och miljön, till exempel förlust av biologisk mångfald, utsläpp av växthusgaser, förgiftSeveral studies have found that organic farms support higher biodiversity, but results vary between studies such that some also show no or even negative effects. There can be many reasons why studies comparing organic and conventional farming show conflicting results. The efficacy of organic farming can depend on focal taxa, landscape context, study scale and the proportion of organically managed

Popular Abstract in Swedish Denna avhandling handlar om antikroppar. Antikroppar är proteiner som bildas av vårt immunförsvar för att skydda oss mot farliga ämnen i vår omgivning, som tex bakterier. På grund av antikroppars unika uppbyggnad och funktion utnyttjas ofta dessa på konstgjord väg. De kan injiceras i kroppen för att bota någon sjukdom eller användas på laboratoriet i olika analyser. InnIn vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. This points

This paper illustrates a novel method to analyze artificial neural networks so as to gain insight into their internal functionality. To this purpose, the elements of a feedforward-backpropagation neural network, that has been trained to detect edges in images, are described in terms of differential operators of various orders and with various angles of operation.

In this study, a knit cotton fabric skin and a Gore-tex skin were used to simulate two sweating methods. The Gore-tex skin was put on top of the pre-wetted knit cotton skin on a dry heated thermal manikin 'Tore' to simulate senseless sweating, similar to thermal manikins 'Coppelius' and 'Walter'. Another simulation involved the pre-wetted fabric skin covered on top of the Gore-tex skin in order to

Syftet med denna framställning är att aktivera en diskussion kring öppna och dolda värden i gruppforskning. Med sådana värden avses här explicit eller implicit uttryckta önskvärda tillstånd i grupper. Det kan röra sig om t.ex. "effektivitet", "ideala samverkanssituationer", "demokrati" eller "arbetsgrupp enligt Bion". För att undersöka förekomsten av sådana genomgicks samtliga bidrag av till grupp

In an organization where the safety level is high and incidents and serious accidents are very rare, a steady erosion of safety awareness and protective measures can occur, especially when productive demands rise. Thus, it is important to continuously identify and monitor aspects in the organization that can affect work performance and safety. In an ongoing joint research project between the LFV G

In order to investigate whether previous findings of ubiquitous skin papillomavirus infection in Caucasians apply to populations from other parts of the world, skin swab samples from Bangladesh, Japan, Ethiopia and Zambia were analysed in parallel with Swedish samples. The prevalence of HPV DNA in the material from Bangladesh was 68 %, Japan 54 %, Ethiopia 52 %, Zambia 42 % and Sweden 70 %. A grea

The gas-to-aqueous solution shifts of the O-17 and C-13 NMR isotropic shielding constants for the carbonyl chromophore in formaldehyde and acetone are investigated. For the condensed-phase problem, we use the hybrid density functional theory/molecular mechanics approach in combination with a statistical averaging over an appropriate number of solute-solvent configurations extracted from classical

We explore here, through three complementary experiments on virtual objects, how intimate active relations with multisensory audio-visual and haptics perceptions allow to the cognitive creation of new believable and plausible objects that can be different of the virtual ones objectively implemented. The three experiments are based on "Pebble Boxes" and consist in the exploration and the manipulati

Recent reports focusing on virulence factors of periodontal pathogens implicated proteinases as major determinants of remarkable pathogenicity of these species, with special emphasis on their capacity to modulate complement activity. In particular, bacteria-mediated cleavage of C5 and subsequent release of C5a seems to be an important phenomenon in the manipulation of the local inflammatory respon