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A general method to directly obtain the DNA sequence of the variable regions of any immunoglobulin chain using a mixture of oligomer primers and the polymerase chain reaction (PCR) is described. Mixed oligonucleotide primers corresponding to the 5′ signal peptide and a conserved 3′ constant region primer were used for enzymatic amplification of each of the heavy and light chain variable regions of
Prothrombin time (PT) is clinically important and is used to monitor oral anticoagulant therapy. To obtain PT results in international normalized ratio (INR), the current standardization procedure is complex and involves reference reagents. The PT of diluted plasma samples can be determined with a combined thromboplastin (the Owren-type procedure), but not necessarily with a plain thromboplastin (
The study considers relations in a group of 95 managers, 41 women and 54 men, between managerial type and signs of anxiety and defense in the Defense Mechanism Technique modified (DMTm). Eight managerial types were distinguished, based on ratings of the managers by their subordinates in terms of the Ekvall and Arvonen (1991) leadership style dimensions of employee-centeredness, change-centeredness
We report on large-scale ab initio calculations for intercombination lines in Si I-like ions. Two measurable quantities, the lifetime of the 3s3p(3 5)S(2) level and the branching ratio of the two lines are discussed, to infer the importance of different features of our calculations. The effects of core polarization, two-body spin-dependent operators and Dirac contra Breit-Pauli approaches are disc
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Soil chemistry under the Solling clean-rain roof was simulated using the dynamic multi-layer soil chemistry model SAFE, including sulfate adsorption. Soil was sampled in order to parameterize the pH and sulfate concentration dependent sulfate adsorption isotherm used in SAFE. Modeled soil solution chemistry was compared to the 14 year long time-series of monthly measurements of soil solution data
3,4-Dihydroxyphenyl-(L)-alanine ((L)-DOPA)-induced dyskinesia is associated with molecular and synaptic plasticity in the basal ganglia, but the occurrence of structural remodeling through cell genesis has not been explored. In this study, rats with 6-hydroxydopamine lesions received injections of the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) concomitantly with (L)-DOPA for 2 weeks. A large
BACKGROUND: To describe the frequency, in some European populations, of the World Health Organisation (WHO) defined metabolic syndrome and to compare the frequency of this syndrome with an alternative definition for non-diabetic subjects, called the insulin resistance syndrome proposed by the European Group for the Study of Insulin Resistance (EGIR). METHODS: Investigators of eight European studie
We have examined the ability of KW-6002, an adenosine A2a antagonist, to modulate the dyskinetic effects of l-DOPA in 6-hydroxydopamine-lesioned rats. In animals rendered dyskinetic by a previous course of l-DOPA treatment, KW-6002 did not elicit any abnormal involuntary movements on its own, but failed to reduce the severity of dyskinesia when coadministered with l-DOPA. A second experiment was u
Popular Abstract in Swedish I den här doktorsavhandlingen presenteras ett datorprogram som utvecklats för att simulera och utvärdera biotekniska processer, där flera processteg kan vara integrerade. Speciellt har en process för framställning av mjölksyra från vetemjöl studerats. Mjölksyra har traditionellt använts i kemi-, läkemedels- och livsmedelsindustrin som t.ex. råvara, surgörare eller konseBiotechnical processes are often integrated to increase productivity and yield. It is difficult to predict the effects of process integration and to find optimum conditions for the process. A valuable tool for the evaluation of a complete process is mathematical modelling. In this thesis, a computer program developed for simulation and optimisation of integrated biotechnical processes is presented
The electronic structure of high-quality CrO2(100) films was investigated by means of x-ray absorption and resonant photoemission spectroscopy at the 2p-3d excitation threshold. The obtained binding energy of the occupied Cr 3d states is in agreement with the results predicted within the local spin-density approximation using the dynamical mean-field theory [L. Craco , Phys. Rev. Lett. 90, 237203
The effect of moderate hypoglycemia (p-glucose, 2.0 +/- 0.3 mmol/L; mean +/- SD) on regional cerebral blood flow (rCBF) was studied in a group of 10 healthy, right-handed men (aged 23 to 28 years) using an intravenous xenon 133 single photon emission computed tomography technique (SPECT). After 10 minutes of hypoglycemia, global CBF had increased to 46.3 +/- 9.6 mL/100 g/min compared with the init
In the present study the incidence of reflux, stricture formation and changes in glomerular filtration rate in patients with the submucosal tunnel or the Camey-Le Duc technique of ureteric implantation into the caecum/detubularized right colon used for continent cutaneous diversion/orthotopic bladder substitution was investigated. Reflux was found in two renal units and ureterointestinal stenosis
PURPOSE: The aim of this study was to investigate the outcome of aortic valve replacement (AVR) and the effect on quality of life in patients aged over 85 who had symptomatic aortic stenosis. METHODS: We performed a retrospective analysis of 21 patients, aged 85-91 years (mean age 86.5), who underwent AVR, 10 of whom underwent concomitant coronary artery bypass grafting (CABG) between 1989 and 199
Popular Abstract in Swedish Denna avhandling behandlar utvecklingen av den norsk-svenska freden mellan 1905-1940. Efterhand kom denna relation att närma sig Stabil Fred—ett förhållande karaktäriserat av avsaknaden av militärt våld, hot om militärt våld, och förväntningar på hot om militärt våld. Utvecklingen mot Stabil Fred smammanfaller delvis med den svenska demokratiseringen, och författaren uThis study concerns the development of Stable Peace between states in the modern anarchical international system. As a starting point for the inquiry the author argues that the prevalence of this type of interstate relationships—characterized by the mutual expectations that neither military violence nor threats thereof will be employed—may be seen as a fundamental challenge to the validity of prev
Bacterial diversity of the mucosal biopsies from human jejunum, distal ileum, ascending colon and rectum were compared by analysis of PCR-amplified 16S rDNA clone libraries. A total of 347 clones from the mucosal biopsies were partially sequenced and assigned to six phylogenctic phyla of the domain Bacteria: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Verrucomicrobia, and Actinobacter
