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Protein Engineering Allows for Mild Affinity-based Elution of Therapeutic Antibodies

Presented here is an engineered protein domain, based on Protein A, that displays a calcium-dependent binding to antibodies. This protein, ZCa, is shown to efficiently function as an affinity ligand for mild purification of antibodies through elution with ethylenediaminetetraacetic acid. Antibodies are commonly used tools in the area of biological sciences and as therapeutics, and the most commonl

Recent advances in the mechanisms and treatment of immune thrombocytopenia

Primary immune thrombocytopenia is an autoimmune disease associated with a reduced peripheral blood platelet count. The phenotype is variable with some patients suffering no bleeding whilst others have severe bleeding which may be fatal. Variability in clinical behaviour and treatment responses reflects its complex underlying pathophysiology. Historically the management has relied heavily on immun

Measuring Mindfulness: Pilot Studies with the Swedish Versions of the Mindful Attention Awareness Scale and the Kentucky Inventory of Mindfulness Skills.

The present article describes data from pilot studies with the Swedish versions of the Mindful Attention Awareness Scale (MAAS) and the Kentucky Inventory of Mindfulness Skills (KIMS). The MAAS and two of the KIMS scales, Act with Awareness and Accept without Judgment, were found to correlate in the predicted direction with measures of well-being and emotional distress. The KIMS scales Observe and

Developing a Code of Ethics for the Swedish audit profession 1923 to 1994 - Juxtaposing the internal and external role of the Code

The paper presents an analysis of context within which FAR, the Swedish professional association representing accountants has developed and revised the Code of Ethics from 1923 to 1995. The purpose is to enhance our understanding of the choices made as to what ethical rules were verbalized or codified and the motivations behind the codification. Building on Birkett and Evans’ (2005) model for orga

Modelling antibody orientation at bacterial surfaces

Varför gör vissa bakterier oss sjuka men inte andra? Hur kommer det sig att vissa bakterier lyckas överleva och infektera särskilda delar av kroppen men väldigt sällan andra? Bakterier och människor har evolverat länge tillsammans och har därmed lyckats utveckla specifika angrepps och försvarsmekanismer mot varandra. Det är känt sedan tidigare att några bakterier uttrycker proteiner med förmågan Bacteria and humans have co-evolved since their common existence. They have thereby developed very specific defence and target mechanisms against one another. Understanding the complex biomolecular processes behind these interactions can be of great use for developing treatments against bacterial diseases. The aim of this thesis project was to verify, and introduce a model for understanding, the i

Redox chemistry of a dimanganese(II,III) complex with an unsymmetric ligand: Water binding, deprotonation and accumulative light-induced oxidation

A dinuclear manganese complex {[(Mn2L)-L-II,IIII(mu-OAc)(2)]-ClO4} has been synthesised, where L is the dianion of 2-{[bis-(pyrid-2-ylmethyl)amino]methyl}-6-{[(3,5-di-tert-butyl-2- hydroxybenzyl)(pyrid-2-ylmethyl)amino]methyl)-4-methylphenol, an unsymmetric binucleating ligand with two coordinating phenol groups. The two manganese ions, with a Mn-Mn distance of 3.498 angstrom, are bridged by the t

TRIPS and Technology Transfer

Abstract in UndeterminedTRIPS is a trade-off between the interest of the developed world in having its technology respected and the need for technology transfer especially to the least-developed countries. But this balance of interests is poorly expressed in the Agreement, which is primarily devoted to protection of intellectual property rights and only marginally addresses the other side of the c

Using Program Analysis to Identify the Use of Vulnerable Functions

Open-Source Software (OSS) is increasingly used by software applications. It allows for code reuse, but also comes with the problem of potentially being affected by the vulnerabilities that are found in the OSS libraries. With large numbers of OSS components and a large number of published vulnerabilities, it becomes challenging to identify and analyze which OSS components need to be patched and u