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Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

Kan hemoglobin från växter lösa humana problem? Dagligen tar vi för givet att vårt blod syresätts tillräckligt för att orka med alla sysslor. Detta möjliggörs av det livsviktiga proteinet hemoglobin som finns i vårt blod. I dagens samhälle råder det dock blodbrist samtidigt som många drabbas av järnbrist. Det är där forskningen på växthemoglobin kommer in i bilden. Experimenten som genomförts i dThe protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. A few years ago, genes that code for Pgb in sugar beet were found and ident

Podcasting as a tool for empowerment. A case study on I Weigh

This thesis would like to explore how people who are part of marginalized groups share their experiences through the podcast format and analyze how people, who do not necessarily identify themselves with one or all the groups discussed in the podcast, relate to the information shared by the guest on the platform. Podcasts can provide essential insights into how marginalized groups use technology t

Den pålitlige tjänstemannen - En tvärdisciplinär undersökning om domstolars hantering av polisers utsagor

I de flesta brottmål som handläggs i svenska domstolar utgör den viktigaste bevisningen utsagor från personer som bevittnat eller blivit utsatta för brott. Dessa utsagor kan i samband med att de avges inför rätten präglas av olika felkällor som på olika vis kan leda till felaktiga utpekanden vilket i längden kan resultera i att oskyldiga döms. Denna uppsats har för avsikt att undersöka sådana felkIn most criminal law cases handled by Swedish courts the most valuable evidence is the one received from cross-examinations of persons who have witnessed a crime or from those who have been subject to a crime. During the cross-examinations, when the testimonies are given, these can be subject to several sources of error which can result in illegitimate statements of guilt which lead to wrongful co

High resolution imaging observations of exoplanet host stars

Stjärnor sägs vara astronomins tidskapsel, genom att studera stjärnor kan vi bättre förstå många fenomen. Stjärnor är en viktig del av vårt universum från det att de skapas till det att de dör. De berikar vårt universum med olika grundämnen som krävs för att planeter ska bildas och för att liv ska uppstå. Det verkar då självklart varför man vill undersöka och förstå alla aspekter kring stjärnbildnThis is a thesis project, analysing data from high-resolution imaging observations, specifically speckle imaging, as a follow up study of exoplanet host stars observed with K2, the second operational phase of the space telescope Kepler. The project was accomplished at NASA Ames research center under the guidance of Rachel A. Matson, during an internship in collaboration with the Swedish National S

Monte Carlo-simulering av tillverkningskostnaden

DPM is a manufacturing company within the metal cutting industry. A number of years ago there emerged a desire to make statistical calculations for its production. Until today, the company has not done any post-calculations, which means that they have based their cost calculations and sales prices on precalculated costs. The absence of post-calculations leads to lack of cost-consciousness that is

"Vi har det bra här" - om omsorg, självbestämmande och inflytande på ett äldreboende. Äldreomsorg sett utifrån ledningens, personalens och de äldres perspektiv.

The purpose of this study has been to explore how different actors in a nursing home (i.e. Head of Unit, staff and residents) argue about elder residents’ care, autonomy and possibilities to influence and how this correlate with the increasing tendencies to view elder people as independent individuals. The study was based on eight qualitative interviews with two Heads of Unit, two staff members an

Klimatanpassad byggnad för varma somrar och kalla vintrar

Outdoor climate has considerable impact on the indoor climate and it is therefore important to adapt the buildings to the climate. This thesis shows how to improve indoor climate in a climate which it is hot, dry in the summer and cold in the winter. My investigative unit is a middle class house in the town Baalbek in Lebanon and the purpose of the study is to come up with proposals on possible so

Semivarians, ett användbart riskmått eller endast ett överflöd?

Since Markowitz presented the mean-variance model as a way of putting together a financial portfolio, variance has been the established measure of risk. Even though the measures of downside risk hasn’t gained the same attention as variance, it has been used in research for some time. Semivariance, best described as the risk of a portfolio’s return falling below a set target, was introduced as a ri

International Epidemiology of Child and Adolescent Psychopathology II: Integration and Applications of Dimensional Findings From 44 Societies

Objective: To build on Achenbach, Rescorla, and Ivanova (2012) by (a) reporting new international findings for parent, teacher, and self-ratings on the Child Behavior Checklist, Youth Self-Report, and Teacher's Report Form; (b) testing the fit of syndrome models to new data from 17 societies, including previously underrepresented regions; (c) testing effects of society gender, and age in 44 societ

Pharmacokinetics of Levodopa, Carbidopa, and 3-O-Methyldopa Following 16-hour Jejunal Infusion of Levodopa-Carbidopa Intestinal Gel in Advanced Parkinson's Disease Patients

Motor complications of Parkinson's disease (PD) are a consequence of pulsatile dopaminergic stimulation from standard oral levodopa therapy. Levodopa-carbidopa intestinal gel (LCIG) is infused continuously via an intrajejunal percutaneous gastrostomy tube. This was the first study designed to characterize the full pharmacokinetic profiles of levodopa, carbidopa, and levodopa metabolite, 3-O-methyl

Consequences of Traffic Casualties in Relation to Traffic-Engineering Factors - An Analysis in Short-term and Long-term Perspectives

Popular Abstract in Swedish Nollvisionen antagen av Sveriges riksdag fastlägger att ingen skall behöva dödas eller skadas svårt i trafiken i Sverige. Införande av detta begrepp förskjuter fokus från trafikolyckorna till konsekvenserna av trafikolyckorna och härmed skapas förutsättningar för ett paradigmskifte i trafiksäkerhetsarbetet. För att åstadkomma önskade förändringar är det nödvändigt och bThe over-arching objectives of this thesis were to formulate a method to describe the consequences of traffic casualties, and to explain the influence of different traffic engineering factors on these consequences for society and individuals over time. A more detailed objective was to examine whether available short-term indicators can be used to predict consequences in a long-term perspective. Ot

BDNF, impulsiveness and avoidant focused coping in suicide attempters

Brain-derived neurotrophic factor (BDNF) is an important protein for neuroplasticity and neurogenesis. In this thesis the role of BDNF, in suicidal behaviour was investigated with focus on possible risk factors for suicidal behaviour such as avoidant focused coping, dysfuntional personality traits like impulsiveness and hyperactivity of the Hypothalamic-Pituitary-Adrenal (HPA) axis.Paper I: The as

Terpenoid Plant Metabolites - Structural Characterization and Biological Importance

In response to the challenges of their local environments, organisms produce of a large number of chemical diverse compounds with complex stereochemistry and reactive functional groups. These characteristics enable them to interact and bind specifically to biological target molecules and exert various biological activities, and have assured that Natural products continues to be an important source

Knowledge, Practices, and Restrictions Related to Menstruation among Young Women from Low Socioeconomic Community in Mumbai, India.

The main objective was to assess knowledge, practices, and restrictions faced by young women regarding their menstrual hygiene. The views of adult women having young daughters were also included and both views were compared. In addition, the factors influencing the menstrual hygiene practices were also studied. The study was carried out during 2008 in Mumbai, India. The mixed methods approach was

Primary adherence to antidepressant prescriptions in primary health care: a population-based study in Sweden.

Background Medical adherence is important in the treatment of depression. Primary medical adherence, i.e. patients collecting their newly prescribed medications from pharmacies, is very different depending on the drug prescribed Objective To assess the rate of primary medical adherence in patients prescribed antidepressants and to identify characteristics that make patients less likely to pick up

The prognostic value of Ki67 is dependent on estrogen receptor status and histological grade in premenopausal patients with node-negative breast cancer.

The aim of this study was to evaluate the prognostic value of Ki67 in relation to established prognostic factors in lymph node-negative breast cancer, and furthermore, whether the prognostic impact was dependent on estrogen receptor (ER) status and histological grade. In 200 premenopausal patients, with 5 years of follow-up, Ki67 was determined on tissue microarrays. In univariate analysis, Ki67 (

Relative sea level changes and glacio-isostatic modelling in the Beagle Channel, Tierra del Fuego, Chile : glacial and tectonic implications

The Beagle Channel crosses the southernmost tip of South America (Tierra del Fuego), connecting the South Atlantic with the Southeastern Pacific. Raised beaches occur up to 10 m above mean sea level (m a.m.s.l.), especially along the northern (Argentinian) shore, and have been dated using marine shells. The southern (Chilean) shore is well-known for its abundance of shell middens at different leve