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The forces between hydrophobic surfaces with physisorbed DNA are markedly and irreversibly altered by exposure to DNA/cetyltrimethylammonium bromide (CTAB) mixtures. In this colloidal probe atomic force microscopy study of the interactions between a hydrophobic polystyrene particle and an octadecyl-trimethylethoxysilane-modified mica surface in sodium bromide solutions, we measure distinct changes
Manual cartographic generalization is a simultaneous process. However, most automatic approaches so far have been sequential; generalization operators are applied one at a time in a certain order. This has been the case both for model generalization (generalization of the conceptual model) and graphic generalization. Our research seeks to demonstrate that the graphic part of cartographic generaliz
Background: Studies on the temporal variation in stroke incidence have reported inconsistent results. Few have studied the temporal variations in case fatality. No study on incidence and case fatality of stroke by season in Sweden has been found. This study explores the weekly, monthly and seasonal variations in incidence and 28-day case fatality of stroke. Methods: A total of 7,129 patients with
Core-level spectroscopy and valence band photoelectron spectroscopy were used to study H2O adsorption on the Si(100) and Ge(100) surfaces. We find that H2O dissociates into H and OH on both surfaces at 300 K. The H and OH are adsorbed in on top positions on the surface. The OH group is tilted with respect to the surface normal on the Si(100) surface. We consider two possible interpretations for th
Scattered vasoactive intestinal polypeptide (VIP)-immunoreactive nerves were found in the striated muscle of the hind limb of the cat, where they usually were associated with small blood vessels. VIP-immunoreactive nerves were also demonstrated in the sciatic nerve; after ligation an abundance of intensely immunoreactive VIP fibres were seen proximal to the ligation. Intraarterial infusion of VIP
It is essential to label roads, landmarks, and other important features on maps for mobile applications to help users to understand their location and the environment. This paper aims to examine real-time map labelling methods suitable for the small screen on mobile devices. A slider method with a continuous search space was proposed to sequentially label both line and point features. The method s
1. We examined the temporal and spatial heterogeneity of zooplankton in lake surface waters during the spring of 3 years in Lake Washington, U.S.A., a large lake with a high production of sockeye salmon fry. 2. We show large within-season and among-year variation in the horizontal distribution of temperature, chlorophyll a concentration, and zooplankton in the lake. The main pattern, a delay in zo
Deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate, and plays important roles in nucleotide metabolism and DNA replication. The dUTPase gene of the retrovirus equine infectious anemia virus (EIAV) was cloned and overexpressed in Escherichia coli using the T7 RNA polymerase expression system. The recombinant vector (pET
An integrative approach to managing host–parasite interactions that threaten species communities will benefit from identifying variation in parasite impact across host species, and host–parasite responses to annual climatic variation. We examine interannual, inter- and intraspecific variation in Philornis downsi intensity – an introduced blood sucking fly that causes high fitness costs in Darwin’s
In an earlier study, we found that Swedish-speaking children with specific language impairment (SLI) differ from younger normally developing compatriots on only a subset of verb morphemes characteristically problematic for children with this type of disorder. In the present study, this finding was replicated, using a carefully devised set of tasks to elicit attempts at the target morphemes. The ch
The structure, stability, and hydrolysis characteristics of beta-lactoglobulin (LG) A are different from those of either beta-LG B or beta-LG C. Purified samples of these proteins were hydrolyzed with trypsin and the rates of loss of native monomeric beta-LG structure were measured using sodium dodecyl sulfate PAGE. At the same time, the appearance of many individual peptides were identified and f
Offspring locomotor performance has been shown to influence fitness related traits in a wide range of taxa. One potential mechanism by which viviparous animals can increase the performance (e.g. sprint speed) of their offspring is by prolonging pregnancy (beyond that required for complete development). However, to date studies examining this potentially important maternal effect have been largely
Although the calcineurin inhibitors (CNI) cyclosporine (CsA) and tacrolimus are highly effective immunosuppressants, they are associated with serious side effects. There is great interest in immunosuppressive regimens that permit reduction or elimination of CNIs, while maintaining adequate immunosuppression and acceptable acute rejection rates. Patients (n = 536) receiving their first renal allogr
The content of the globular domains G1, G2 and G3 on the core protein of high-density (A1D1) aggrecan isolated from newborn and mature bovine cartilage and from cultures of bovine chondrocytes was examined. Quantitation based on the 220 nm absorbance of tryptic marker peptides from each domain isolated by reversed-phase HPLC showed that while the content of G1 and G2 was essentially the same for a
After birth, the gastrointestinal (GI) tract undergoes vast structural and functional adaptations to be able to digest mother's milk and later, during the weaning period, solid food. Studies on germ-free animals have shown the role of the gut microbiota for stimulating GI maturation, but which groups are involved is unclear. In the present study, we administered the probiotic bacterium, Lactobacil
