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We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a
Due to the migration megatrend, efficient and effective second-language acquisition is vital. One proposed solution involves AI-enabled conversational agents for person-centered interactive language practice. We present results from ongoing action research targeting quality assurance of proprietary generative dialog models trained for virtual job interviews. The action team elicited a set of 38 re
This work presents a novel concept for acoustofluidic cell manipulation: thin vibrating channel walls. Improved etch recipes enable us to produce thin walls between two microfluidic channels, fabricated in silicon or via PDMS casting, that can be used for challenging microbiological applications. We demonstrate the medium exchange for bacteria utilised for transformation, single-cell rotation for
In comparison to other human cancer types, malignant melanoma exhibits the greatest amount of heterogeneity. After DNA-based detection of the BRAF V600E mutation in melanoma patients, targeted inhibitor treatment is the current recommendation. This approach, however, does not take the abundance of the therapeutic target, i.e., the B-raf V600E protein, into consideration. As shown by immunohistoche
Detection and classification of distresses is a fundamental activity in the road pavement management. Even in the early stages of deterioration, road pavement needs to be monitored to identify problems, evaluating the actual conditions and predicting what the future conditions will be. Monitoring activities through manual/visual inspections are time consuming, costly and cause of safety concerns.
Structural Health Monitoring (SHM) can provide valuable information for maintenance-related activities and post-disaster emergency management. However, as with any technological system, SHM systems can be susceptible to errors due to malfunctioning. Therefore, it is essential to assess the potential for malfunctions and the associated costs of maintenance and repair when evaluating the long-term b
Avian Malaria infects thousands of species of birds across the Aves class. The most widespread (geographically and phylogenetically)morphological species is Plasmodium relictum, and of that, the mitochondrial lineage of SGS1 is the most common and invasive. It infects146 bird species, with large differences in infection outcome, from low intensity chronic disease to shorter, highly virulent infect
Since 2011, LUNARC has aimed to provide an interactive HPC environment for its resource users. Several different architectures have been used, but since 2013, we have been using a remote desktop environment based on Cendio's ThinLinc [1] combined with a custom backend framework, GfxLauncher [2], supporting hardware-accelerated graphics applications and Jupyter Notebooks [3] submitted to the backen
Precision cut lung slices (PCLS) are complex three-dimensional (3-D) lung tissue models, which preserve the native microenvironment, including cell diversity and cell-matrix interactions. They are an innovative ex vivo platform that allows studying disease as well as the effects of therapeutic agents or regulatory molecules [e.g., microRNA (miRNA)]. The aim of our study was to develop a protocol t
Therapeutic resistance and recurrence remain core challenges in cancer therapy. How therapy resistance arises is currently not fully understood with tumors surviving via multiple alternative routes. Here, we demonstrate that a subset of cancer cells survives therapeutic stress by entering a transient state characterized by whole-genome doubling. At the onset of the polyploidization program, we ide
The identification of helium nuclei at LHCb is achieved using a method based on measurements of ionisation losses in the silicon sensors and timing measurements in the Outer Tracker drift tubes. The background from photon conversions is reduced using the RICH detectors and an isolation requirement. The method is developed using pp collision data at √s = 13 TeV recorded by the LHCb experiment in th
The aim of ADINE project is to develop and to demonstrate new methods for the electric distribution network management including distributed generation (DG). The active network management (ANM) method needs enabling solutions such as protection, voltage and reactive power control and planning and information systems of networks. The actual devices and monitored data from the real-life demonstratio
Fluorescence techniques for applications in tissue diagnostics and therapy monitoring were investigated and developed in the work presented in this thesis. The aim was to develop fluorescence diagnostics for the detection of malignant tumors and better delimitation of the tumor margins, using the enhanced contrast in fluorescence between tumor and surrounding healthy tissue. Fluorescence may be ge
Popular Abstract in Swedish Avloppsvatten renas från fosfor för att minimera utsläppet till sjöar och hav. Näringsämnena fosfor och kväve bidrar till övergödningen i våra vatten och kan reduceras kraftigt på avloppsreningsverken. Fosforavskiljning går huvudsakligen ut på att få löst fosfat att hamna i en fast fas ? slammet, för att sedan kunna avskilja det från det renade vattnet. Bakterier som vThe idea of phosphorus removal at wastewater treatment plants is to transfer dissolved phosphorus into a solid phase. Together with the rest of the produced sludge it is then removed from the water, resulting in a decreased concentration of phosphorus in the effluent and the prevention of the eutrophication in the recipient. Phosphorus can either be stored biologically, with enhanced biological p
Al2O3 and HfO2 high-k oxides deposited by atomic layer deposition have been studied on InAs 100 and 111B surfaces. By using a low-frequency fitting routine, interface defect densities have been extracted. In general, both HfO2 and Al2O3 show similar D-it profiles, with a D-it minimum around 2x10(12) eV(-1)cm(-2).
Five sets of gloves were tested on 3 hand models with 2 measuring system in order to ensure repeatability of results from the past and for the future. The other aims of the study were to compare the possible differences related to different number of measuring zones, and effect of temperature distribution over hand surface. The hand models had 2 (HF), 9 (H2) and 10 (H3) zones respectively. Models
Popular Abstract in Swedish Dagens storskaliga tillverkning baseras på varor med relativt kort livstid och lågt pris. Vinsterna för tillverkarna kommer från den stora mängden sålda varor. Det finns dock inom många industribranscher krympande vinstmarginaler och många företag söker därför efter nya källor för att uppnå bättre rörelseresultat. Även miljöfrågorna är en utmaning för industrin. VäxandeLife cycle environmental problems have been addressed by a number of strategies. However, the results are mostly lamentable because solutions are searched for within the same paradigms that give rise to the problems. This research questions the vision of linear material growth and searches for ways to sustain economic growth without putting the natural environment under stress. The research explor
Treatment resistant depression (TRD) and suicidal behavior are among the most important public health problems and are commonly associated with significant disability and psychosocial impairment. Although there have been recent advances in identifying neurobiological correlates of these complex conditions, their pathophysiology still remains unclear. Although the recent advances concerning the neu
This paper presents an acoustofluidic chip for the sorting of bacteria from protozoa in water using standing acoustic waves. The size-based acoustophoretic sorting method provides a means for sample pre-concentration, which is vital for real-time, on-site monitoring of drinking water quality and preventing outbreak of waterborne diseases. In practice, high throughput and high recovery pre-concentr
Popular Abstract in Swedish Människors sätt att visualisera objekt bygger på en del underliggande principer som man kanske inte alltid reflekterar över. Det faktum att vi har just två ögon gör det till exempel möjligt för oss att avgöra på vilket avstånd ett objekt befinner sig. Detta kallas för parallaxeffekten. Kameror, som bara har ett "öga", saknar denna förmåga, vilket försvårar avbildande mäLaser sheet imaging, also known as planar laser imaging, is one of the most versatile optical imaging techniques known and is frequently applied in several different domains. It furthermore constitutes the basis for a variety of other 2D methods, in turn allowing visualization of e.g. velocity, particle size, species concentration and temperature. However, when applied on turbid, light scattering
