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Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, Cumbersome and require a large number of cells, making it difficult to study rare cell types Such its certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, auto
This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint te
Ovarian cancer is usually found at a late stage when the prognosis is often bad. Relative survival rates decrease with tumor stage or grade, and the 5-year survival rate for women with carcinoma is only 38%. Thus, there is a great need to find biomarkers that can be used to carry out routine screening, especially in high-risk patient groups. Here, we present a large-scale study of 64 tissue sample
We demonstrate that the a. chain of human C4b binding protein (C4BP) binds directly to CD40 on human B cells at a site that differs from that used by CD40 ligand. C4BP induces proliferation, upregulation of CD54 and CD86 expression, and IL4-dependent IgE isotype switching in normal B cells but not in B cells from patients with CD40 or IKKgamma/NEMO deficiencies. Furthermore, C4BP colocalized with
Multiple sclerosis (MS) and other T cell-mediated autoimmune diseases develop in individuals carrying a complex susceptibility trait, probably following exposure to various environmental triggers. Owing to the presumed weak influence of single genes on disease predisposition and the recognized genetic heterogeneity of autoimmune disorders in humans, candidate gene searches in MS have been difficul
Bladder Exstrophy-Epispadias Complex (BEEC), the severe end of the uro-rectal malformation spectrum, has a profound impact on continence as well as sexual and renal functions. It is widely accepted that for the majority of cases the genetic basis appears to be multifactorial. Here, we report the first study which utilizes genome-wide association methods to analyze a cohort comprising patients pres
Background: Chromosomal aberrations in breast tumors from BRCA1 and BRCA2 germ-line mutation carriers are considerably more frequent than what is seen in sporadic breast tumors. According to Comparative Genomic Hybridisation analysis (CGH), deletions on chromosome 4 are one of the most frequent events in BRCA1-associated tumors, suggesting inactivation of specific tumor suppressor genes. Materials
Background: Bisphosphonates are effective agents for the management of osteoporosis. Their low bioavailability and low potency necessitate frequent administration on an empty stomach, which may reduce compliance. Gastrointestinal intolerance limits maximal dosing. Although intermittent intravenous treatments have been used, the optimal doses and dosing interval have not been systematically explore
Acute promyelocytic leukaemia (APL; AML M3) is identified by a unique t(15;17) translocation which fuses the PML gene to the retinoic acid receptor alpha gene (RARA). Reverse transcription coupled with the polymerase chain reaction (RT-PCR) has been used to develop a diagnostic test for APL based on the PML-RARA fusion message. Separate PCR assays were designed to amplify either PML-RARA (15q+ der
Previous work suggests N-methyl-D-aspartate receptor (NMDAR) activation may be involved in degeneration of medium-sized spiny striatal neurons in Huntington's disease (HD). Here we show that these neurons are more vulnerable to NMDAR-mediated death in a YAC transgenic FVB/N mouse model of HD expressing full-length mutant huntingtin, compared with wild-type FVB/N mice. Excitotoxic death of these ne
The European hummingbird hawkmoth Macroglossum stellatarum is a diurnal nectar forager like the honeybee, and we expect similarities in their sensory ecology. Using behavioural tests and electroretinograms (ERGs), we studied the spectral sensitivity of M. stellatarum. By measuring ERGs in the dark-adapted eye and after adaptation to green light, we determined that M. stellatarum has ultraviolet (U
Given electronic wave functions generated by the grasp2K relativistic atomic structure package, this program calculates diagonal magnetic dipole A(J) and electric quadrupole B-J hyperfine interaction constants and Lande g(J) factors. In addition the program computes diagonal and off-diagonal reduced hyperfine and Zeeman matrix elements and constructs the total interaction matrix for an atom in an
We present a novel, interferometric, two-color, high-order harmonic generation setup based on a turn-key Ytterbium-doped femtosecond laser source and its second harmonic. Each interferometer arm contains a spatial light modulator with individual capabilities to manipulate the spatial beam profiles and to stabilize the relative delay between the fundamental and the second harmonic. In addition, sep
Background: Japan lacks efficient assessment tools for occupational balance (OB) in people with mental illness (MI). Aims/objectives: We investigated the test–retest reliability and construct validity of a Japanese version of the Satisfaction with Daily Occupations and Occupational Balance (SDO-OB-J) for people with MI. Methods: SDO-OB-J interviews were conducted twice at a 2-week interval. Result
En metod för att studera cellers vandringsbeteenden genom automatiserad mikroskopi Vår kropp byggs upp av flera olika celler som fyller specifika funktioner. Många celler måste kunna förflytta sig för att utföra sina funktioner. Våra organ formas under fostrets utveckling genom att celler förflyttar sig och immunförsvarets celler vandrar genom kroppen för att jaga och bekämpa smittämnen. Cellers Automated fluorescence microscopy is an emerging technique that enables researchers to generate large amounts of data which in turn allows for large scale quantitative studies. In previous small scale quantitative studies, migration modes found by qualitatively observing migrating lung cancer cells have been described. Since such a method starts from qualitative observations it might not generaliz
Tort liability for inducement of breach of contract was introduced to English law already in the mid-1800s, through the case of Lumely v Gye. In Swedish law, however, there were until quite recently only very limited legal support for such a liability. But that changed in 2005 when the Supreme Court of Sweden pronounced its decision in NJA 2005 s. 608. This essay describes the conditions under whi
Immune checkpoint inhibitors (ICIs), targeting checkpoint receptors such as programmed cell death protein 1 (PD-1), are associated with increased risk of cardiovascular events, but the underlying mechanisms remain poorly understood. Here we show that PD-1 + T cells from murine atherosclerotic aortas mainly display a progenitor exhausted phenotype (PD-1 intSlamf6 +Tim3 -), produce IFNγ in vivo, exh
OBJECTIVE: To synthesise evidence related to the dissemination and implementation (D&I) of injury prevention interventions for female, woman and/or girl (female/woman/girl) athletes.DESIGN: Scoping review.DATA SOURCES: MEDLINE, APA PsycInfo, Cochrane Databases for Systematic Review, Cochrane Central Register for Controlled Trials Registry, EMBASE, CINAHL, SPORTDiscus, ERIC, ProQuest Dissertati
Introduction: Despite factor (F)VIII prophylaxis, a perceived increased risk of bleeding for some people with severe haemophilia A (PwSHA) exists, limiting physical activity (PA) and restricting quality of life (QoL). Aim: HemiNorth 2 (EudraCT# 2020-003256-32) is an interventional study evaluating the impact of switching from FVIII prophylaxis to emicizumab in PwSHA without FVIII inhibitors who ha
The 7Be(d, α)5Li and 7Be(d, p)8Be*(p 7Li) reactions are studied at 5 MeV/u in the context of the cosmological lithium problem. This work aims to probe the contribution of 7Be destruction mechanisms, particularly through channels that populate intermediate unbound states of 5Li and 8Be. The contribution of 7Be(d, α)5Li(p α) reaction is separated from the 7Be(d, p)8Be*(2α) reaction. The 7Be(d, p)8Be
