Filetype
Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and
Microglia are activated in many neurological diseases and have been suggested to play an important role in the development of affective disorders including major depression. To investigate how microglial signaling regulates mood, we used bidirectional chemogenetic manipulations of microglial activity in mice. Activation of microglia in the dorsal striatum induced local cytokine expression and a ne
AIMS: There is considerable controversy regarding optimal management of patients with paraesophageal hiatus hernia (pHH). This survey aims at identifying recommended strategies for work-up, surgical therapy, and postoperative follow-up using Delphi methodology.METHODS: We conducted a 2-round, 33-question, web-based Delphi survey on perioperative management (preoperative work-up, surgical procedure
Docent | Universitetslektor Kontaktinformation E-post: julie [dot] hassing_nielsen [at] svet [dot] lu [dot] seOrganisation Statsvetenskapliga institutionen Hämtställe: 31 WebbplatsJulie Hassing Nielsens profil i Lunds universitets forskningsportalAndra roller Universitetslektor Statsvetenskapliga institutionenSubjectPolitical Science - Statsvetenskap Main research areasComparative PoliticsPolitica
https://www.svet.lu.se/julie-hassing-nielsen - 2026-07-09
This thesis has contributed to the methodological development in the field of assistive technology and has generated new knowledge about the effectiveness and the service delivery process when powered mobility devices are provided to adult first time users in a Nordic context. Some of the results have been used to investigate the relationship between the three key elements of Donabedian’s conceptu
Popular Abstract in Swedish Inom forskningsområdet biogeokemi studerar man hur naturens olika grundämnen, såsom kol, kväve, fosfor och kisel ändrar form och transporteras genom landskapet för att via bäckar och vattendrag till slut nå havet. Tillsammans beskriver dessa olika processer, som sker på olika tidsskalor, från sekunder till flera miljoner år, ett grundämnes kretslopp (eller biogeokemiskaSilicon is the next most abundant element in the Earth’s crust and its biogeochemical cycle is linked with that of carbon. Further, silicon is a beneficial nutrient for plants in terrestrial ecosystems and a key nutrient for diatoms in aquatic ecosystems. During the last decade the important role of terrestrial vegetation in controlling Si fluxes downstream aquatic environments, via incorporation
The aim of the study was to visualize design regulations of furniture by means of interactive technology based on earlier studies and practical examples. The usage of the visualized regulations was evaluated on two occasions: at the start when the first set of regulations was presented, and after six years of usage of all regulations. The visualized regulations were the result of a design process
Twelve years ago, eleven European countries voluntarily abandoned their home currencies and joined the Euro. The founding fathers of the Euro believed that creation of the common currency would strengthen integration among the members and catalyze economic convergence progress within the common currency area. This study examines this issue in detail. I focus on labor productivity convergence and s
This was a cross-sectional study investigating factors related to satisfaction with care among long-term mentally ill patients diagnosed with schizophrenia, selected from an outpatient register. Demographic factors, personality variables, and health-related factors were related to their satisfaction with care. Satisfaction with care showed no relationships to demographic factors such as age, livin
Cervical dystonia is a neurological movement disorder causing abnormal posture of the head. It may be accompanied by involuntary movements which are sometimes tremulous. The condition has marked effects on patients' self-image, and adversely affects quality of life, social relationships and employment. Botulinum neurotoxin (BoNT) is the treatment of choice for CD and its efficacy and safety have b
We report the development of a high-level bacterial expression system for the Alzheimer's disease-associated amyloid beta-peptide (A beta), together with a scaleable and inexpensive purification procedure. A beta(1-40) and A beta(1-42) coding sequences together with added ATG codons were cloned directly into a Pet vector to facilitate production of Met-A beta(1-40) and Met-A beta(1-42), referred t
The aim was to investigate how patients in a clinical setting, combining acute stroke care and rehabilitation, perceived the value and meaning attached to a commonly used instrumental occupation, namely "Brewing coffee and making an open-face cheese sandwich with sliced vegetable". Another aim was to validate the ValMO model, proposing that value and meaning are related phenomena. Upon performing
Popularized summary Nowadays, the growing prevalence of natural disasters is driving people to pay more and more attention to emergency management. Progress in catastrophe analysis capabilities based on Geographical Information System (GIS) may allow the needs of public participation to be considered. Synchronous data sharing between citizens and emergency workers could effectively promote the proScientific summary The increasing prevalence of natural disasters is driving people to pay more and more attention to emergency management. Progress in catastrophe analysis capabilities based on Geographical Information System (GIS) may allow the needs of public participation to be considered. Synchronous data sharing between citizens and emergency workers could effectively promote the process of
BackgroundResearch is limited on registered healthcare professionals (RHCP) usage of research and evidence-based practice (EBP) in Swedish municipal primary healthcare work.Aim/ObjectivesThe aim of this study was to increase the understanding of experiences, attitudes, and conditions of usage of research and implementation of EBP among RHCPs in a Swedish municipality setting. Further, the study ai
