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An integrated process for purifying a 6.1 kilo base pair (kbp) plasmid from a clarified Escherichia coli cell lysate based on an ultra/diafiltration step combined with polymer/polymer aqueous two-phase system and a new type of chromatography is described. The process starts with a volume reduction (ultrafiltration) and buffer exchange (diafiltration) of the clarified lysate using a hollow fibre me
Pålning är en typ av grundläggning lämplig för att grundlägga broar vid svaga markförhållanden. Att manuellt utforma en pålgrupp är en iterativ, tidskrävande process där snittkrafterna i pålarna måste verifieras för ett stort antal lastfall. Efter installationen kan pålarna dessutom ha nya positioner och kapaciteten måste verifieras igen. I detta projekt utvecklades en mjukvara för att automatiserFor bridges in weak ground conditions, piling is an appropriate type of foundation. To manually arrange the piles in a pile group is an iterative, time consuming process where the section forces in the piles must be verified for a large number of load cases. In addition, after installation, piles may have new positions and the capacity must be re-verified. In this thesis, a software was developed
BACKGROUND: Definition of temporal serum proteome profiles after out-of-hospital cardiac arrest may identify biological processes associated with severe hypoxia-ischaemia and reperfusion. It may further explore intervention effects for new mechanistic insights, identify candidate prognostic protein biomarkers and potential therapeutic targets. This pilot study aimed to investigate serum proteome p
Characterising the stop/start walking process of individuals in a crowded and congested space is an important consideration in modelling pedestrian movement. However, the reaction of pedestrians to speed changes, especially to the person in front, has not been fully characterised nor quantified for full adoption in computer models. This study, therefore, explored the different phases of the stop/s
The aim of this work was to develop a model of an integrated biomass-to-liquid process, consisting of hydrothermal liquefaction, evaporation, gasification and Fischer-Tropsch synthesis, using lignocellulosic forest residues as feedstock to produce hydrocarbons suitable for upgrade into drop-in biofuels. The energy, mass and carbon efficiencies that were achieved were 35%, 20% and 32%, respectively
CreditsEach course or programme is measured in credits, using the European Credit Transfer and Accumulation System (ECTS).60 ECTS credits are equal to the workload of a full-time student over one academic year (i.e. two semesters of study over 40 weeks).30 ECTS credits are equal to the workload of a full-time student over half an academic year (i.e. one semester of study over 20 weeks).One credit
https://www.ace.lu.se/education/about-masters-programme-asian-studies/credits-and-grading-system - 2026-07-07
The paper describes a simple and efficient control loop decoupler for TITO (two inputs, two outputs) control systems. The inverted decoupling approach is used, and the feedforward filters are just static gains. The feedforward gains are determined in two ways, from a static analysis and from optimization. By investigating several cases found in literature, it is found that the two sets of feedforw
Medications are of considerable help if healthcare providers are able to administer them to patients safely and appropriately. The medication use process is complex and a team of professionals – doctors, nurses, and pharmacists – work together with the patient for optimal treatment effect. The process involves many sub-processes and in every step there is a possibility for an error. Cytotoxic drug
The gas-to-aqueous solution shifts of the O-17 and C-13 NMR isotropic shielding constants for the carbonyl chromophore in formaldehyde and acetone are investigated. For the condensed-phase problem, we use the hybrid density functional theory/molecular mechanics approach in combination with a statistical averaging over an appropriate number of solute-solvent configurations extracted from classical
For numerous spin crossover complexes, the anisotropic distortion of the first coordination shell around the transition metal center governs the dynamics of the high-spin/low-spin interconversion. However, this structural parameter remains elusive for samples that cannot be investigated with crystallography. The present work demonstrates how picosecond X-ray absorption spectroscopy is able to capt
