Search results

Efficient screening of ligands interacting with G-protein-coupled receptors is central for modern drug development. Here, we describe an optimized reporter vector primarily intended for use in reporter cell lines expressing such receptors. The construct consists of a synthetic enhancer containing 9x TRE (12-O-tetradecanoylphorbol-13-acetate-responsive elements) fused to a minimal CMV (cytomegalovi

The lifetimes of three short-lived levels belonging to the 4f(9) 6p configuration and of two long-lived levels of the 4f(9) 5d configuration of Dy iii have been measured for the first time using time-resolved laser-induced fluorescence techniques. They are in good agreement with theoretical values calculated within the framework of a pseudo-relativistic configuration interaction approximation. Usi

PRR recognize conserved structures on pathogenic microbes and are important for the defense against invading microorganisms. However, accumulating evidence indicates that many pathogens have evolved mechanisms to avoid recognition by PRR. One type of PRR is the macrophage scavenger receptor A (SR-A), which has been shown to play an important role in recognition and non-opsonic phagocytosis of path

In developing countries such as Ethiopia, where chronic gastritis and peptic-ulcer disease are the most common endoscopic findings, it is important to study the association between Helicobacter pylori infection and gastroduodenal diseases. Both invasive and non-invasive diagnostic methods were therefore used to investigate 300, consecutive, adult patients with dyspepsia, from the gastrointestinal

The efficacy of a short (Group I) and a prolonged (Group II) course with ciprofloxacin was assessed in patients undergoing transurethral prostatic resection for benign hyperplasia or cancer of the prostate and compared with that of controls without antibiotic (Group III). Both regiments significantly reduced the frequency of postoperative bacteriuria (p less than 0.01) and of severe infectious com

We investigated the role of the major histocompatibility complex (MHC) region in the specificity of autoimmunity by analysing specifically the development of sialadenitis, but also insulitis, nephritis and autoantibody production in autoimmune-prone nonobese diabetic (NOD) mice where the MHC H2g7 haplotype had been exchanged for the H2q (NOD.Q) or H2p (NOD.P) haplotype. The exchange of H2 haplotyp

IL-8 is a major human neutrophil chemoattractant at mucosal infection sites. This study examined the C-X-C chemokine response to mucosal infection, and, specifically, the role of macrophage inflammatory protein (MIP)-2, one of the mouse IL-8 equivalents, for neutrophil-epithelial interactions. Following intravesical Escherichia coli infection, several C-X-C chemokines were secreted into the urine,

Alltsedan det kalla kriget slutade har försvarssektorn, framförallt militär logistik och försvarsmaterielanskaffning, genomgått en genomgripande transformation. Flera faktorer har bidragit till transformationen: förändringar i länders och organisationers försvars- och säkerhetspolitik; reducerade försvarsbudgetar; deltagande i fredsbevarande och fredsframtvingande operationer; erfarenheter från deSince the ending of the Cold War, the defence sector, particularly the areas of military logistics and defence acquisition, has been undergoing a comprehensive transformation. There are several factors that explain this transformation: changes in defence and security policies for nations and organisations; reductions in defence expenditure; participation in Peace Support Operations; Lessons Learne

This paper presents the incorporation of an auto-tuning real-time garbage collector into a feedback-based on-line scheduling system. The studied feedback scheduler is designed to dynamically adjust the sampling periods of a set of controller tasks in order to maximize the overall control performance. In the suggested approach, the memory management overhead is made explicit and taken into account

We address the question of which syntactic representation is best suited for role-semantic analysis of English in the FrameNet paradigm. We compare systems based on dependencies and constituents, and a dependency syntax with a rich set of grammatical functions with one with a smaller set. Our experiments show that dependency-based and constituent-based analyzers give roughly equivalent performance

The Swedish county council Region Skane is developing work processes for iterative risk and vulnerability analyses. This will be arranged in a distributed bottom-up manner, where analyses are performed and results reported through three hierarchical levels, ending at a central unit within the county council organisation. That unit is responsible for three tasks: 1) Establish a summary of the risks

In order to identify the protein responsible for a dopamine peroxidizing activity, previously described in human normal and parkinsonian substantia nigra by our group, we developed non-denaturing polyacrylamide gel electrophoresis conditions, mimicking the characteristic colour in vitro reaction, resulting from cyclic oxidation of dopamine (DA). After separating protein mixtures from human normal

Human C4b-binding protein (C4BP) is a soluble, multiple-subunit inhibitor of complement that circulates in blood. Recently C4BP was shown to bind DNA, reduce DNA release from necrotic cells and limit DNA-mediated complement activation in solution. Herein we employed nuclear magnetic resonance spectroscopy to measure chemical shift perturbations and used them to restrain the computational docking o

Magnesium (Mg) is an abundant metal which has been used in aviation, medicine, hydrogen energy storage, etc. However, Mg can be rather reactive, and therefore an improved understanding of corrosion and oxidation mechanisms can enhance the efficiency of these processes to control and widen applications. The study presented here investigates the mechanisms of oxidation from the initial to full monol