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Currently, in real-time PCR, one often has to choose between using a sequence-specific probe and a nonspecific double-stranded DNA (dsDNA) binding dye for the detection of amplified DNA products. The sequence-specific probe has tire advantage that it only detects the targeted product, while the nonspecific dye has the advantage that melting curve analysis can be performed after completed amplifcat
In addition to the basic regulatory functions, a batch control system must support production planning and scheduling, recipe management, resource allocation, batch report generation, unit supervision and exception handling. A closed-loop framework is presented in this work that integrates decision support tools required at the different levels of a decision-making hierarchical batch control syste
Heparan sulfate chains have been found to be associated with amyloid deposits in a number of diseases including transmissible spongiform encephalopathies. Diverse lines of evidence have linked proteoglycans and their glycosaminoglycan chains, and especially heparan sulfate, to the metabolism of the prion protein isoforms. Glypicans are a family of glycosylphosphatidylinositol-anchored, heparan sul
There are three relativization strategies in the Austroasiatic lang Kammu: (1) deletion of the relativized NP, (2) deletion & change of word order, & (3) pronominalization of the relativized NP. The use of these strategies for the six positions (subject, direct O, indirect O, oblique NP, genitive, & O of comparison) on the relativization hierarchy proposed by E. Keenan & B. Comrie
We present an extensive investigation of the epitaxial growth of Au-assisted axial heterostructure nanowires composed of group IV and III-V materials and derive a model to explain the overall morphology of such wires. By analogy with 2D epitaxial growth, this model relates the wire morphology (i.e., whether it is kinked or straight) to the relationship of the interface energies between the two mat
The potential of measuring temperature and multiple species concentrations (N-2, O-2, CO) by use of combined vibrational coherent anti-Stokes Raman spectroscopy (CARS) and pure rotational CARS has been investigated. This was achieved with only one Nd:YAG laser and one dye laser together with a single spectrograph and CCD camera. From measurements in premixed sooting C2H4-air flames it was possible
LiBH4 is one of the promising candidates for hydrogen storage materials because of its high gravimetric and volumetric hydrogen capacity. However, its high dehydrogenation temperature and limited reversibility has been a hurdle for its use in real applications. In an effort to overcome this barrier and to adjust the thermal stability, we make a composite system LiBH4-Ca(BH4)(2). In order to fully
Event-related potentials (ERPs) were recorded from 13 scalp sites during the performance of an associative recall task. At study, subjects were presented with a series of word pairs and were required to incorporate the two members of each pair into a sentence. At test, the first members of each pair were presented intermixed with an equal number of unstudied items. Subjects were required to discri
This paper re-examines the validity of the monetary exchange rate model during the post-Bretton Woods era for 18 OECD countries. Our analysis simultaneously considers the presence of both cross-sectional dependence and multiple structural breaks, which have not received much attention in previous studies of the monetary model. The empirical results indicate that the monetary model emerges only whe
Purpose The aim of this study was to evaluate the feasibility of using 1-[C-11]-acetate positron emission tomography (ACE-PET) to detect and delineate the gross tumour volume of head and neck cancer before radiotherapy, and to compare the results with those obtained using F-18-fluoro-2-deoxy-D-glucose (FDG) PET. Methods Ten patients with histologically verified squamous cell carcinoma were investi
The new prominence given to science for economic growth and industry comes with an increased policy focus on the promotion of commodification and commercialization of academic science. This paper posits that this increased interest in commodification is a new steering mechanism for governing science. This is achieved by first outlining what is meant by the commodification of scientific knowledge t
1 In an asexually reproducing plant (Potamogeton pectinatus L.) the competitive advantage of a larger propagule over a smaller one was determined by a single parameter (alpha, the competitive asymmetry coefficient), independent of sowing density or average propagule size. Competitive advantage was determined by the relative size difference between propagules instead of their absolute difference. 2
The polygyny threshold model was introduced in the 1960s in order to explain why females in some species choose to mate vith already mated males. Since then, a number of complementary or alternative hypotheses have been suggested. By using a hierarchical testing approach Searcy and Yasukawa (1989) organised the models that proposed to explain the maintenance of territorial polygyny. Here, I point
Objective: To compare the effects of low-dose transdermal estradiol (E2)/norethisterone acetate (NETA) patches (Estalis 25/125) with low-dose oral E2/NETA (Activelle) on cardiovascular biochemical markers after 12 and 52 weeks of treatment in postmenopausal women with intact uteri. Design: Participants were randomly assigned to receive either transdermal E2/NETA (delivering daily doses of 25 [mu]
Purpose We tested whether total prostate-specific antigen velocity (tPSAv) improves accuracy of a model using PSA level to predict long-term risk of prostate cancer diagnosis. Methods During 1974 to 1986 in a preventive medicine study in Sweden, 5,722 men aged <= 50 gave two blood samples about 6 years apart. We measured free (fPSA) and total PSA (tPSA) in archived plasma samples from 4,907 partic
Sex determination is a fundamental process governed by diverse mechanisms. Sex ratio selection is commonly implicated in the evolution of sex-determining systems, although formal models are rare. Here, we argue that, although sex ratio selection can induce shifts in sex determination, genomic conflicts between parents and offspring can explain why single-factor systems (e.g. XY/XX or ZW/ZZ) are co
The partial phase diagram of D2O/n-octyl-beta-D-alkyl-glucoside(C(8)G(1))/n-octane has been determined at T = 25 degrees C. The diagram contains a funnel-shaped micellar phase originating from the water corner of the phase diagram D2O/C(8)G(1) with the stern forming a narrow three-phase region, in which the three phases in equilibrium are two microemulsions of similar composition and an excess oil
Na+/H+ exchangers are ubiquitous in mammalian cells, carrying out key functions, such as cell volume defense, acid-base homeostasis, and regulation of the cytoskeleton. We used two screening technologies (FLIPR and microphysiometry) to characterize the signal transduction pathway used by the bradykinin beta(2) receptor to activate Na+/H+ exchange in two cell lines, KNRK and CHO. In both cell types
The isothermal pseudo-ternary-phase diagram was determined at 25 degreesC for systems composed of lecithin, water, and, as oil, either isooctane or decane. This was accomplished by a combination of polarizing microscopy, small-angle X-ray scattering, and NMR techniques. The lecithin-rich region of the phase diagram is dominated by a lamellar liquid-crystalline phase (L-alpha). For lecithin content
