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We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that
A theoretical framework for understanding public library development is presented; plural agonistics is used in combination with an approach inspired by post-humanism. The framework is related to examples from a study of two Swedish public libraries. Antagonism is viewed as foundational, and democracy as an ongoing process, the task being to transform antagonism to agonism, with institutions like
Treatment resistant depression (TRD) and suicidal behavior are among the most important public health problems and are commonly associated with significant disability and psychosocial impairment. Although there have been recent advances in identifying neurobiological correlates of these complex conditions, their pathophysiology still remains unclear. Although the recent advances concerning the neu
This paper presents an acoustofluidic chip for the sorting of bacteria from protozoa in water using standing acoustic waves. The size-based acoustophoretic sorting method provides a means for sample pre-concentration, which is vital for real-time, on-site monitoring of drinking water quality and preventing outbreak of waterborne diseases. In practice, high throughput and high recovery pre-concentr
We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known
In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DN
Amyloid beta peptides (Aβ) proteins play a key role in vascular pathology in Alzheimer’s Disease (AD) including impairment of the blood–brain barrier and aberrant angiogenesis. Although previous work has demonstrated a pro-angiogenic role of Aβ, the exact mechanisms by which amyloid precursor protein (APP) processing and endothelial angiogenic signalling cascades interact in AD remain a largely un
Common remediation and land management approaches often preclude economically attractive and low risk brownfield redevelopment. The authors argue that recently developed dynamic site characterization techniques can greatly improve sound decision making on land revitalization options. A number of these in-situ soil and groundwater test methods, such as MIP, ROST™/UVOST™, XRF and BAT, based on geote
The changes in extracellular Ca2+ (Cae) and K+ (Ke) activities were studied in the rat brain during insulin-induced hypoglycemia. At about the time of onset of isoelectric EEG in severe insulin-induced hypoglycemia (300-g male Wistar rats under 70% N2O anaesthesia), there was an increase in Ke which, at ∼13 mM, was associated with a fall in Cae. Ke peaked at 48 ± 12 mM, and Cae at 0.18 ± 0.28 mM.
In this paper, a distinctly subjectivist analysis of the nature of relational goodness or goodness for is proposed. Like the generic subjectivist analysis of value, the proposal is to analyse value in terms of attitudes. Specifically, the proposed analysis of goodness for appeals to a special kind of attitude: namely, so-called for-someone’s-sake attitudes. Unlike other analyses in the literature
Structural Health Monitoring (SHM) can provide valuable information for maintenance-related activities and post-disaster emergency management. However, as with any technological system, SHM systems can be susceptible to errors due to malfunctioning. Therefore, it is essential to assess the potential for malfunctions and the associated costs of maintenance and repair when evaluating the long-term b
Enligt författarnas mångåriga erfarenhet från kustprojekt runt om i Sverige föreligger ett stort behov av en förbättrad terminologi på svenska vad gäller ingenjörsmässiga frågeställningar och planering av kustområden.Föreliggande artikel presenterar således en engelsk-svensk ordlista, inklusive förklarande kommentarer, med syfte att klargöra en lång rad ord och uttryck. Denna finns också att tillg
This work focuses on the investigation on both local and global dielectric properties for a random wound single tooth winding, which represents a segment of the in-field electrical machine used on an Hybrid Wheel Loader. Finite Element Analysis (FEA) is used to predict the temperature stress while loading and illustrate the influences of dielectric properties (i.e. conductivity and dielectric cons
4MOST,1 the 4m Multi-Object spectrographic Survey Telescope, is an upcoming optical, fiber-fed, MOS facility for the VISTA telescope at ESO's Cero Paranal Observatory (Chile). The preliminary design of 4MOST features 2,400 fibers split into a low-resolution (1,600 fibers, 390-900 nm, R > 5; 000) and a high-resolution channel (800 fibers, three arms, ∼20-25 nm coverage each, R > 18; 000) with an Ec
We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dy
We present the first one-step acoustic separation of high quality plasma and mononuclear cells (MNCs) from undiluted human whole blood. This enables centrifugation-free integration in analytical instrumentation.
Implementing electrically connected metal-coated posts in a Deterministic Lateral Displacement (DLD) device and applying electric fields, we use electrokinetics to achieve tunable particle separations and to trap and manipulate DNA. The strength of DLD stems from its typically binary behavior. Particles move in one out of two trajectories based on their effective size. For particles that are close
We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing,
