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Human monoclonal antibodies against a recombinant HIV envelope-antigen produced by primary in vitro immunization. Characterization and epitope mapping.

Peripheral blood lymphocytes from healthy, HIV sero-negative blood donors have been in vitro immunized using penv9, a recombinant fragment of the envelope of HIV-1. This primary in vitro immunization followed by Epstein-Barr virus (EBV) transformation and somatic cell fusion subsequently gave rise to several specific anti-penv9 monoclonal antibodies (MO28, MO30 and MO43) of μ isotype. The hybridom

Real time analysis of antibody-antigen reaction kinetics.

Surface plasmon resonance, i.e. detection of changes in refractive index on a surface, was used in a biosensor to evaluate the dissociation/association rate and affinity constants of human monoclonal IgG and IgM antibodies and Tab fragments. The results showed that an observed difference in affinity constants between intact and fragmented IgG anti-tetanus antibody was related to approximately 10-f

Forgotten by Many and Remembered by Few’ : Religious Responses to Forced Migration in Georgia

This chapter examines the institutional dimensions of religious responsesto forced migration in contemporary Georgia. It focuses on the internallydisplaced population of Georgia from the two breakaway territories ofAbkhazia and South Ossetia/Tskhinvali region to further scrutinise howmajor religious organisations respond to the signifijicant migration crises onthe level of policy and discourse. The

Moisture Levels in Historic Timber Walls Insulated with Hemp-Lime

The European Union has a low-carbon roadmap that aims at reducing greenhouse gas emissions toprevent further climate change and achieve climate-neutrality by 2050. Many of the historical buildings that wehave today will still be around in 2050. Hence, there is a need to improve their energy performance. A way to dothis is to use hemp-lime as a material for renovation. Hemp shiv, the woody core par

Differentiating properties of occupancy rate and workload to estimate crowding : A Swedish national cross-sectional study

BACKGROUND: Emergency department (ED) crowding causes increased patient morbidity and mortality. ED occupancy rate (OR; patients by treatment beds) is a common measure of crowding, but the comparability of ORs between EDs is unknown. The objective of this investigation was to investigate differences in ORs between EDs using staff-perceived workload as reference.METHODS: This was a national cross-s

Rich or Lean? : A phenomenological alternative for explaining early social cognition

Abstract in ItalianUn’accesa e radicale tensione nelle scienze cognitive è quella tra il cognitivismo e il cosiddetto approccio delle quattro E (Embodied, Enactive, Embedded e Extended). La mancanza di una riflessione aperta e sostanziale sul lavoro teorico ed empirico proposto dall’approccio opposto appare problematica da entrambe le parti. Il presente contributo analizza a fondo le evidenze empiIn philosophy and cognitive science, the tension between cognitivism and the 4E-Cognition approach is both deep and polarizing. A lack of serious engagement with the theoretical and empirical work generated by the opposing framework seems problematic on both sides. In this paper, we closely discuss data on early socio-cognitive development produced by an influential nativist current of thought in

The attractions of Salafi-jihadism as a gendered counterculture: Propaganda narratives from the Swedish online “sisters in deen”

By conducting an online ethnographic study of the unofficial propaganda narratives posted on Facebook by Swedish women affiliated with the Salafi-jihadi movement, Dr Henriette Frees Esholdt explores and analyses the female-specific attractions of Salafi-jihadism within a Swedish context in Chapter 4. In addition to the promotion of Sharia law, gender segregation, as well as the maintenance of a pa

Creaky voice in South Swedish accent 1

Pitch and voice quality are increasingly understood as closelyintertwined. While Swedish and Norwegian word accents have traditionally been understood in terms of pitch, Danish stød, which is systematically related when it comes to distribution and function, has been described as a type of creaky voice. According to the Laryngeal Articulator Model (LAM), both pitch lowering and creaky or harsh voi

A new software engineering programme - structure and initial experiences

Software plays an increasingly important role in new products of different kinds. Therefore, the need of engineers developing software is continuously increasing. However, computer science education programmes are not enough to fulfill the industrial needs. Software engineering programmes are requested with a holistic approach to the software life cycle and its economics as well as education in mo

Mothers ́ experiences in relation to a new Swedish postnatal home-based model of midwifery care - Prospective cross sectional study

Background:The goal of postnatal care is to provide the highest possible quality of care and medical safety with the least possibleintervention in order to optimize health and wellbeing of the new family. The aim of the study was to describemothers´ experiences in relation to a new postnatal home-based model of midwifery care.Method:Prospective cross sectional study based on quantitative and quali

Are fewer circumference measurements reliable to determine volume measurements in lower limb lymphedema compared to every 4th cm?

Objective: In lymphedema management, lower limb volume is commonly assessed by circumference measurementevery 4th cm which is however time-consuming. The aim of this study is to evaluate the agreement between volumemeasurements derived from circumference measurements every 4th cm (V4) with every 8th cm (V8) and every 12th cm(V12) in patients with lower limb lymphedema (LLL) and to determine the te

Collagen-induced arthritis development requires alpha beta T cells but not gamma delta T cells: studies with T cell-deficient (TCR mutant) mice

Collagen type II (CII)-induced arthritis (CIA) in mice is a model for rheumatoid arthritis (RA) in which the role of T lymphocytes remains controversial. To clarify this, we have bred a targeted gene deletion of TCR beta or delta loci into two mouse strains susceptible to CIA, the B10.Q and DBA/1 strains. The TCRbeta-/- mice lacked alphabeta T cells, which was compensated by an expansion of B cell

Non-Major Histocompatibility Complex dependent variations in lymphocyte activity between inbred mouse strains susceptible to various autoimmune diseases

Transgenic techniques in inbred mouse strains are powerful tools to investigate the specific roles of genes in biological pathways and disease models. However, there is increasing concern over the influence of a variable genetic background in such experiments. To date there have been few investigations of the immunological differences between inbred mouse strains used in models of autoimmune disea

Dynamics of atomic oxygen production in an NH3/air flame assisted by a nanosecond pulsed plasma discharge

Atomic oxygen (O) is vital in plasma-assisted combustion, but its quantitative measurement is challenging and therefore rarely reported. This work performs femtosecond two-photon-absorption laser-induced fluorescence (fs-TALIF) imaging to quantify the dynamics of atomic oxygen production by a single nanosecond (ns) pulsed plasma discharge in an ammonia/air flame for the first time. First of all, t

Unpacking (Ir)regular Labour Migration

This chapter unpacks the complex and contested nature of ‘irregular labour migration’ and explores the meaning and analytical rigour of this conceptualisation. We discuss four social positions: 1) authorised migrant regular worker, 2) authorised migrant irregular worker, 3) unauthorised migrant irregular worker and 4) unauthorised migrant regular worker. Using the distinctions between informalisat