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Increased population longevity could be influenced by early life factors. Some areas have long-lived populations, also in a historical perspective. We aimed to study these factors in Halland, an area with the highest life expectancy in Sweden. We collected archival data on gestational age and birth characteristics from 995 live singleton full-term births at the Halmstad Hospital, Halland, from the

There is emerging evidence of an association between epigenetic modifications, glycemic control and atherosclerosis risk. In this study, we mapped genome-wide epigenetic changes in patients with type 2 diabetes (T2D) and advanced atherosclerotic disease. We performed chromatin immunoprecipitation sequencing (ChIP-seq) using a histone 3 lysine 9 acetylation (H3K9ac) mark in peripheral blood mononuc

During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). In the adult, HSC quiescence, maintenance, and differentiation are closely linked to changes in metabolism. However, metabolic processes

Background: The relationship between growth differentiation factor 15 (GDF-15) and the development of chronic kidney disease (CKD) is still unclear. We sought to examine whether plasma GDF-15 was related to incident CKD and kidney function decline using a large prospective cohort study. Methods: 4318 participants of the Malmö Diet and Cancer Study-Cardiovascular Cohort were examined in 1991-1994.

OBJECTIVE: Kawasaki disease (KD) is a vasculitis of unknown aetiology with a high risk of coronary aneurysms if untreated. Timely treatment with intravenous immunoglobulin decreases the risk for coronary artery aneurysms (CAA). In this study, we set out to elucidate the factors associated with the risk of developing CAA. METHODS: Records of all KD-diagnosed children in Skåne between 2004 and 2014

Simulating temporal changes in breast tissue has been challenging, despite advancement in virtual clinical trials. This study aims to enable virtual trials over time, motivated by interest in the effect of tissue involution on tumor visibility. We present a framework for creating 3D voxel-based breast phantoms and lesions using in-house Perlin Noise-based algorithm. The framework consists of three

Nanoplastics are emerging environmental contaminants that increasingly threaten soil ecosystems, yet their effects on microbial behavior remain poorly understood. This is mainly due to the lack of experimental tools capable of directly observing microbial dynamics in situ under realistic soil-like conditions. Here, we present a proof-of-concept system that enables real-time, high-throughput monito

Catalytic active phases are often separated from inactive ones by their appearance/removal in heating/cooling experiments. Such experiments can reveal ignition, extinction, and often an associated hysteresis. The corresponding behavior and hysteresis under rapid gas composition changes in the milliseconds regime remain largely unexplored. However, such experiments can potentially be highly rewardi

A cell’s ability to sense and respond to the mechanical properties of the extracellular matrix (ECM) is essential for maintaining tissue homeostasis, and its disruption contributes to diseases such as fibrosis, cardiovascular disorders, and cancer. Effective mechanical coupling between the plasma membrane, the underlying filamentous actin (F-actin) cytoskeleton, and integrin-based adhesion complex

Background: Virtual imaging trials (VITs) are in silico studies that simulate medical imaging and disease processes, offering a cost-effective and reproducible addition to traditional imaging trials. While VITs are well established in breast imaging, most existing implementations simulate imaging based on static anatomical models, capturing only a single time point. This limits their ability to st

The International Agency for Research on Cancer has recently classified occupational exposure as a firefighter as carcinogenic (group 1A). Even though the occupation of firefighter is associated with high exposure to pollutants, it is challenging to carry out exposure studies to assess health risks due to the extreme conditions associated with firefighting. Routine monitoring of occupational expos

BACKGROUND AND AIMS: Atherosclerotic plaques are the leading cause of cardiovascular events. Single-cell approaches have identified diverse human plaque cell phenotypes but their spatial distribution and interactions remain unclear. Here, intercellular communication patterns in human plaque microenvironments were mapped to reveal novel targets to prevent atherosclerotic events.METHODS: Spatial tra

Smart microscopy represents a paradigm shift in biological imaging, moving from passive observation tools to active collaborators in scientific inquiry. Enabled by advances in automation, computational power, and artificial intelligence, these systems are now capable of adaptive decision-making and real-time experimental control. Here, we introduce a theoretical framework that reconceptualises sma

We present a microchannel-based adaptation of mass density-gradient centrifugation for the mass density measurement of cells or microparticles. The workflow consists of three basic steps: Microchannel filling, centrifugation, and microscopy. Microchannel filling and subsequent centrifugation of two liquids with different densities allows the instantaneous, precise, and repeatable generation of a o

Aims: In a co-twin control design, we aimed to examine whether individuals who initiate metformin treatment have an altered risk of incident diagnosis of osteoarthritis (OA), for up to 11 years after starting the treatment, compared with their non-treated co-twin. Methods: In all twin pairs different for metformin treatment (N=1 261 pairs) and all eligible twins/singletons (N=52 341) aged 35–75 ye

BACKGROUND: Ageing is associated with circadian rhythm sleep disorders, poor sleep at night, less physical activity and more time spent indoors, affecting the wellbeing of older adults. Their sleep and mood could benefit from daytime outdoor physical activity, exposure to daylight, better indoor lighting and sleep routines. However, changes to routines can be challenging depending on individual be

Here, we present a workflow for transcriptional silencing of transposable elements (TEs) in human induced pluripotent stem cells (hiPSCs). We describe steps for designing guide RNAs (gRNAs) to target TE families or unique TE loci. We also detail procedures for validating the efficiency and specificity of large-scale CRISPRi-based silencing using a multiome approach combining bulk RNA sequencing, C

AIMS: To build a comprehensive biobank integrated in the Swedish Heart Failure Registry (SwedeHF) comprising comprehensive clinomic data, proteomic, transcriptomic and genomic information in combination with clinical and diagnostic characteristics and additional ICD-code registry data.METHODS: Blood and urine samples will be biobanked at SwedeHF registration with an optional second sampling after

Long-distance migrants face numerous challenges during their migration, with the spring migration timing being particularly important, as early arrival at the breeding grounds often enhances breeding success. Both innate and environmental factors influence migration timing in birds, and the earlier arrival of males than females is common among songbirds breeding in temperate zones. The great reed