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Nearly 60% of the worlds adult population is projected to be overweight or obese by 2030. The prevalence of obesity- related diseases, such as type 2 diabetes (T2D), has increased markedly in the past decades. The adipose tissue plays an important role in disease progression of T2D. Adipocyte insulin sensitivity, adipose tissue’s ability to expand, and accumulation of subcutaneous rather than visc
Seven native residues on the regulatory protein calmodulin, including three key methionine residues, were replaced (one by one) by the vibrational probe amino acid cyanylated cysteine, which has a unique CN stretching vibration that reports on its local environment. Almost no perturbation was caused by this probe at any of the seven sites, as reported by CD spectra of calcium-bound and apo calmodu
Presented here is an engineered protein domain, based on Protein A, that displays a calcium-dependent binding to antibodies. This protein, ZCa, is shown to efficiently function as an affinity ligand for mild purification of antibodies through elution with ethylenediaminetetraacetic acid. Antibodies are commonly used tools in the area of biological sciences and as therapeutics, and the most commonl
Background Monotherapy with anticoagulation has been considered as first-line therapy in patients with mesenteric venous thrombosis (MVT). The aim of this study was to evaluate outcome, prognostic factors, and failure rate of anticoagulation as monotherapy, and to identify when bowel resection was needed.Methods Retrospective study of consecutive patients with MVT diagnosed between 2000 and 2015.RBACKGROUND: Monotherapy with anticoagulation has been considered as first-line therapy in patients with mesenteric venous thrombosis (MVT). The aim of this study was to evaluate outcome, prognostic factors, and failure rate of anticoagulation as monotherapy, and to identify when bowel resection was needed.METHODS: Retrospective study of consecutive patients with MVT diagnosed between 2000 and 2015
Pulmonary artery grafts are needed as cardiovascular bioprosthetics. For successful tissue recellularization after transplantation, lipids have to be removed from the donor artery. Developing a selective process to remove lipids without damaging the extracellular matrix greatly depends on knowing the amount and type of lipid compounds in the specific tissue. Here we present an efficient methodolog
Large-scale land acquisitions are a critical driver of global socio-environmental change, in particular in the Global South. This study aims to investigate drivers, impacts and feedbacks of environmental change in Kilombero Valley, Tanzania, a region in rapid societal and environmental transformation. We use land cover classifications to map and quantify land cover changes from 1990 to 2016, and c
BACKGROUND: Older people with multi-morbidity are major users of healthcare and are often discharged from hospital with ongoing care needs. This care is frequently provided by informal caregivers and the time immediately after discharge is challenging for caregivers with new and/or additional tasks, resulting in anxiety and stress.AIM: This study aimed to describe mental health, with particular re
Hydroxyapatite (HA) ceramic is a widely used synthetic bone substitute material for the regeneration of bone defects. We manufactured HA scaffolds with adjustable pore sizes and pore geometry by dispense-plotting. In addition, we attached peptides covalently onto the HA surface and are able to simultaneously quantify the amount of covalently attached and adsorbed peptide down to the picomolar rang
Favorable propagation (FP) and channel hardening (CH) are desired properties in massive multiple-input multiple-output (MIMO) systems. To date, these properties have primarily been analyzed for classical statistical channel models, or ray-based models with very specific angular parameters and distributions. This paper presents a thorough mathematical analysis of the asymptotic system behavior for
The direct reprogramming of adult skin fibroblasts to neurons is thought to be controlled by a small set of interacting gene regulators. Here, we investigate how the interaction dynamics between these regulating factors coordinate cellular decision making in direct neuronal reprogramming. We put forward a quantitative model of the governing gene regulatory system, supported by measurements of mRNA
Advances in synchrotron storage rings and beamline automation have pushed data-collection rates to thousands of data sets per week. With this increase in throughput, massive projects such as in-crystal fragment screening have become accessible to a larger number of research groups. The quality of support offered at large-scale facilities allows medicinal chemistry-focused or biochemistry-focused g
Evacuation models generally tend to make over-simplifications in the representation of human behaviour in fire due to the difficulties to capture its complexity. This work shows how ‘new’ discrete choice model approaches allow overcoming this issue. A description of the existing state-of-the-art of discrete choice models is introduced to highlight the potential of those models. Three case studies
Huntington's disease (HD) is the most prevalent polyglutamine expansion disorder. HD is caused by an expansion of CAG triplet in the huntingtin (HTT) gene, associated with striatal and cortical neuronal loss. Central and peripheral metabolic abnormalities and altered insulin-like growth factor-1 (IGF-1) levels have been described in HD. Thus, we hypothesized that restoration of IGF-1-mediated sign
The thermal unfolding of three SH3 domains of the Tec family of tyrosine kinases was studied by differential scanning calorimetry and CD spectroscopy, The unfolding transition of the three protein domains in the acidic pH region can be described as a reversible two-state process. For all three SH3 domains maximum stability was observed in the pH region 4.5 < pH < 7.0 where these domains unfold at
The planar Hall effect was used for investigation of magnetic anisotropy in short period (Ca,Mn)As/CaAs superlattices epitaxially grown on (001) oriented GaAs substrate. The results confirmed the existence of low-temperature magneto crystalline anisotropy in the superlattices with the easy magnetic axes directed along the two in-plane (100) directions. Attention is paid to the two-state behaviour
Directed and elliptic flow of pi(0) - decay photons in 158 A GeV Pb+Pb collisions has been studied near midrapidity in an analysis of data obtained with the photon spectrometer LEDA of the WA98 experiment at the CERN SPS. Preliminary results on the rapidity and transverse momentum dependence of the flow have been obtained for various centrality classes for p(T) > 0.2 GeV/c and 2.3 < y < 2.9. The r
We have performed x-ray magnetic circular dichroism, x-ray resonant magnetic scattering and scanning tunnelling microscopy measurements on ultrathin Co films deposited on flat and vicinal Cu(111). The range of film thickness varies between one and 25 monolayers. For the vicinal Cu(111), Co deposition below one monolayer leads to the formation of elongated islands preferentially oriented along the
